Tek Rabbit Polyclonal Antibody

CAT#: AP31728PU-L

Tek rabbit polyclonal antibody, Purified

Product Images

Western analysis of recombinant Human and Mouse sTIE-1 and sTIE-2 with a Polyclonal antibody directed against Mouse recombinant sTIE-2. There is a very weak cross reactivity with Human sTIE-2 but not with Human and Mouse sTIE-1 visible.

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  FACS analysis of TIE-2 expression in primary mouse endothelial cells (SnoMec).

Specifications

Product Data

• Applications: FC, WB
• Recommended Dilution: Western Blot: 2-5 μg/ml.
  Flow Cytometry: 1-5 μg/ml.
• Reactivities: Mouse
• Host: Rabbit
• Isotype: IgG
• Clonality: Polyclonal
• Immunogen: Highly pure (>95%) recombinant Mouse soluble TIE-2 (Ala23-Ala737) derived from Insect cells.
• Specificity: Recognizes TIE-2 (CD202b/TEK)
• Formulation: PBS, pH 7.2
  State: Purified
  State: Liquid purified IgG fraction
• Purification: Protein A Chromatography
• Gene Name: Homo sapiens TEK receptor tyrosine kinase (TEK), transcript variant 1
• Database Link:
  Entrez Gene 21687 Mouse
• Background: Recombinant Mouse soluble TIE-1 was fused with a 6x His-tag at the C-terminus. The soluble receptor protein consists of the full extracellular domain (Ser22-Ala748). Mouse sTIE-1 monomer has a calculated molecular mass of approximately 79,8 kDa. As a result of glycosylation, the recombinant protein migrates as an approximately 95 kDa protein in SDS-PAGE under reducing conditions. TIE-1 (tyrosine kinase with Ig and EGF homology domains 1) and TIE-2/Tek comprise a receptor tyrosine kinase (RTK) subfamily with unique structural characteristics: two immunoglobulin-like domains flanking three epidermal growth factor (EGF)-like domains and followed by three fibronectin type III-like repeats in the extracellular region and a split tyrosine kinase domain in the cytoplasmic region.
  These receptors are expressed primarily on endothelial and hematopoietic progenitor cells and play critical roles in angiogenesis, vasculogenesis and hematopoiesis.
• Synonyms: TIE2, TIE-2, Angiopoietin-1 receptor, p140 TEK

Reference Data

• Protein Families: Druggable Genome, ES Cell Differentiation/IPS, Protein Kinase, Transmembrane