ATF 4 (ATF4) Rabbit Polyclonal Antibody

CAT#: AP31750PU-N

ATF 4 (ATF4) rabbit polyclonal antibody, Purified

Product Images

Western Blot analysis of HeLa cell lysates with anti-ATF4 antibody at 1/500 dilutions.

• 

  Human Small Intestine: Formalin-Fixed, Paraffin-Embedded (FFPE).

Specifications

Product Data

• Applications: ELISA, IHC, WB
• Recommended Dilution: ELISA: 1/20000.
  Immunohistochemistry on Paraffin Sections: 2 µg/ml.
  Western Blot: 1/500.
• Reactivities: Human, Mouse, Rat
• Host: Rabbit
• Isotype: IgG
• Clonality: Polyclonal
• Immunogen: Synthetic peptide.
• Specificity: This antibody detects endogenous levels of ATF4 protein. Not tested with other proteins.
• Formulation: PBS, pH 7.4 containing 0.05% Sodium Azide as preservative
  State: Purified
  State: Liquid purified Ig fraction
• Concentration: lot specific
• Storage: Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
  Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.
• Gene Name: Homo sapiens activating transcription factor 4 (ATF4), transcript variant 1
• Database Link:
  Entrez Gene 11911 MouseEntrez Gene 79255 RatEntrez Gene 468 Human
• Background: Activating transcription factor 4 (ATF4) was initially identified as a widely expressed mammalian DNA binding protein that could bind tax-responsive enhancer element within the LTR of HTLV-1. In a later study, it was isolated and characterized as cAMP-response element binding protein 2 (CREB-2). However, this sequence (M86842) has no sequence similarity with the CREB2 gene. ATF4 belongs to a family of DNA-binding proteins including the AP-1 family of transcription factors and cAMP-response element binding proteins (CREBs) and CREB-like proteins. ATF4 gene encodes a 351 amino acid protein that contains a leucine zipper structure (protein-protein interaction) located C-terminal to a stretch of basic amino acids (DNA binding), a characteristic of its family of transcription factors. ATF4 was shown to bind and negatively regulate transcription from the cAMP response element. It is widely expressed in human tissues suggesting that it may be involved in regulating transcription in a wide variety of cell types.
• Synonyms: CREB2, CREB-2, TXREB, Activating transcription factor 4, ATF-4

Reference Data

• Protein Families: Transcription Factors
• Protein Pathways: GnRH signaling pathway, Long-term potentiation, MAPK signaling pathway, Neurotrophin signaling pathway, Prostate cancer