RPS6 (C-term) Rabbit Polyclonal Antibody

CAT#: AP31752PU-N

RPS6 (C-term) rabbit polyclonal antibody, Purified

Product Images

Western blot analysis of extracts from HeLa cells, treated with TNF-a 20ng/ml 2', using antibody. The lane on the right is treated with the synthesized peptide.

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  Human Pancreas: Formalin-Fixed, Paraffin-Embedded (FFPE)

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  Immunofluorescence analysis of HeLa cells, using S6 Ribosomal Protein Antibody. The picture on the right is treated with the synthesized peptide.

Specifications

Product Data

• Applications: ELISA, IF, IHC, WB
• Recommended Dilution: ELISA: 1/1000.
  Immunofluorescence: 1/100 - 1/500.
  Immunohistochemistry on Paraffin Sections: 1/200 - 1/400.
  Western Blot: 1/500 - 1/1000.
• Reactivities: Human, Mouse, Rat
• Host: Rabbit
• Isotype: IgG
• Clonality: Polyclonal
• Immunogen: Synthetic peptide from Human S6 Ribosomal Protein.
  Epitope: C-Terminus.
• Specificity: This antibody detects endogenous levels of total S6 Ribosomal Protein protein.
• Formulation: PBS (without Mg2+, Ca2+), pH 7.4, 150 mM Sodium Chloride, 0.02% Sodium Azide and 50% Glycerol
  State: Purified
  State: Liquid purified Ig fraction
• Concentration: lot specific
• Purification: Immunoaffinity Chromatography
• Storage: Store the antibody (in aliquots) at -20°C.
  Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.
• Gene Name: Homo sapiens ribosomal protein S6 (RPS6)
• Database Link:
  Entrez Gene 20104 MouseEntrez Gene 29304 RatEntrez Gene 6194 Human
• Background: 40S ribosomal protein S6 (also known as RPS6) is a ~31 kDa substrate of p70 S6 kinase (p70S6K) and a major component of translational machinery involved in protein synthesis, cell growth, proliferation, and metabolism. RPS6 undergoes phosphorylation on multiple serines in the carboxyl terminal region in the order 236-->235-->240-->244-->247, due to the positions of these amino acid residues on the alpha-helix. Hyperphosphorylation of RPS6 stimulates protein synthesis that mediates progression through the cell cycle.
• Synonyms: Phosphoprotein NP33, 40S ribosomal protein S6

Reference Data

• Protein Pathways: Insulin signaling pathway, mTOR signaling pathway, Ribosome