IL6R Rabbit Polyclonal Antibody

CAT#: AP31764PU-N

IL6R rabbit polyclonal antibody, Aff - Purified

Product Images

Western Blot analysis of Jurkat and 803 cell lysates with anti-IL6R antibody at 1/200 dilution

• 

  Human Spleen: Formalin-Fixed, Paraffin-Embedded (FFPE)

Specifications

Product Data

• Applications: ELISA, IHC, WB
• Recommended Dilution: ELISA: 1/40000 - 1/80000.
  Immunohistochemistry on Paraffin Sections: 2.5 µg/ml.
  Western Blot: 1/200 - 1/500.
• Reactivities: Human
• Host: Rabbit
• Isotype: IgG
• Clonality: Polyclonal
• Immunogen: Synthetic peptide
• Specificity: This antibody detects Human IL6 Receptor.
• Formulation: PBS (without Mg2+, Ca2+), pH 7.4, 150 mM Sodium Chloride, 0.02% Sodium Azide and 50% Glycerol
  State: Aff - Purified
  State: Liquid purified Ig fraction
• Concentration: lot specific
• Purification: Affinity Chromatography
• Storage: Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
  Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.
• Gene Name: Homo sapiens interleukin 6 receptor (IL6R), transcript variant 1
• Database Link:
  Entrez Gene 3570 Human
• Background: IL6 exerts its activity through binding to a high affinity receptor complex consisting of two membrane glycoproteins: an 80 kDa IL6 binding receptor protein (IL6R) and a 130 kDa signal transducing protein consisting of a 19 amino acid residue signal peptide (gp130). Human IL6R is a 468 amino acid residue type I membrane protein consisting of a 19 amino acid residue signal peptide, a 339 residue extracellular region, a 28 residue transmembrane region and an 82 residue cytoplasmic region. The ectodomain of IL6R is made up of an immunoglobulin like domain and a cytokine receptor superfamily domain. A naturally occurring soluble form of the IL6R, which binds IL6 and which mediates IL6 signaling through interaction with gp130, is identified in the urine of healthy adult humans. It has also been detected in human and mouse serum as well as in the conditioned medium of various cells including human peripheral blood mononuclear cells, and several T cell or granulocyte/macrophage cell lines. Increased expression of the c-fos and c-myc protooncogenes have been linked to IL6 receptor activation.
• Synonyms: Interleukin-6 receptor subunit alpha, IL-6R-alpha, IL-6R 1, IL6 Receptor alpha

Reference Data

• Protein Families: Druggable Genome, ES Cell Differentiation/IPS, Transmembrane
• Protein Pathways: Cytokine-cytokine receptor interaction, Hematopoietic cell lineage, Jak-STAT signaling pathway