MAP2 Chicken Polyclonal Antibody

CAT#: AP31808PU-N

MAP2 chicken polyclonal antibody, Aff - Purified

Product Images

Dissociated cell cultures of an e13 Mouse brain showing MAP-2 (green staining) of neurons. DAPI (blue staining) allows visualization of nuclei.

Specifications

Product Data

• Applications: IF, IHC, WB
• Recommended Dilution: Western Blot.
  Immunocytochemistry.
  Immunohistochemistry.
  Recommended Dilutions:
  1/2000-1/5000 for Western blots.
  1/1000-1/2000 for Immunohistochemistry and Immunocytochemistry using 2% paraformaldehyde-fixed tissues or cells.
  Quality Control: This antibody was analyzed by Immunohistochemistry (at a dilution of 1/2000) using Fluorescein-labeled Goat anti-Chicken IgY (1/500 dilution, Cat.-No AP31795FC-N) as the secondary reagent.
• Reactivities: Human, Mouse
• Host: Chicken
• Isotype: Ig
• Clonality: Polyclonal
• Immunogen: Two synthetic peptides KLH conjugated
  corresponding to different regions of the MAP-2 gene product, but are shared between the Human (NP_002365) and Mouse (P20357) sequences.
  Production: After repeated injections, immune eggs were collected, the IgY fractions were purified from the yolks.
  These IgY fractions were then affinity-purified using a peptide column, and the concentrations of the eluates adjusted to 200 µg/ml. Finally, equal volumes of both of these affinity purified anti-peptide antibodies were mixed, and the preparation was filter-sterilized.
• Specificity: Recognizes MAP-2 (Microtubule-Associated Protein-2).
• Formulation: 10mM PBS, pH 7.2 containing 0.02% Sodium Azide as preservative.
  State: Aff - Purified
  State: Liquid purified (filter sterilized) IgY fraction.
• Concentration: lot specific
• Purification: Affinity Chromatography using a peptide column.
• Storage: Store the antibody undiluted in the dark at 2-8°C.
• Stability: Shelf life: one year from despatch.
• Gene Name: Homo sapiens microtubule associated protein 2 (MAP2), transcript variant 1
• Database Link:
  Entrez Gene 17756 MouseEntrez Gene 4133 Human
• Background: Human MAP-2 is a 199,296 dalton protein (1827 amino acids) expressed in neurons of the PNS and CNS, where it serves as a major component of the neuronal cytoskeleton. MAP-2 contributes to structural integrity and cell shape.
  This gene encodes a protein that belongs to the microtubule-associated protein family. The proteins of this family are thought to be involved in microtubule assembly, which is an essential step in neurogenesis. The exact function of this gene is still unknown. The products of similar genes in rat and mouse are neuron-specific cytoskeletal proteins that are enriched in dentrites, implicating a role in determining and stabilizing dentritic shape during neuron development.
• Synonyms: Microtubule-associated protein 2, MAP2, Neuronal Marker

Reference Data

• Protein Families: Adult stem cells, Druggable Genome, ES Cell Differentiation/IPS