Nes Chicken Polyclonal Antibody

CAT#: AP31810PU-N

Nes chicken polyclonal antibody, Aff - Purified

Product Images

• 

  A tissue section through an e13 Mouse brain showing Nestin (green staining) in neural stem cells of the ventricular zone of the CNS. DAPI (blue staining) allows visualization of nuclei.

Specifications

Product Data

• Applications: IF, IHC, WB
• Recommended Dilution: Western Blot.
  Immunocytochemistry.
  Immunohistochemistry.
  Recommended Dilutions:
  1/2000-1/5000 for western blots.
  1/10000-1/20000 for Immunohistochemistry and Immunocytochemistry using 2% paraformaldehyde-fixed tissues or cells.
  Quality Control: Each of the three antibodies were analyzed by Immunohistochemistry (at a dilution of 1/20000) using Fluorescein-labeled Goat anti-Chicken IgY (1/500 dilution, Cat.-No AP31795FC-N) as the secondary reagent.
• Reactivities: Mouse, Rat
• Host: Chicken
• Isotype: Ig
• Clonality: Polyclonal
• Immunogen: Three synthetic peptides KLH conjugated corresponding to different regions of the Mouse Nestin gene product (NP_057910), but are shared with the Rat (NM_012987) protein sequence.
  Production: After repeated injections, immune eggs were collected, and the IgY fractions were purified from the yolks. These IgY fractions were then affinity-purified using a peptide column, and the concentrations of the eluates adjusted to 0.3 mg/ml. Finally, equal volumes of each of the three affinity-purified anti-peptide antibodies were mixed, and the preparation was filter-sterilized.
• Specificity: Recognizes Nestin.
  Note that the sequences of the Mouse and Human Nestin genes are divergent, and it is unlikely that antibodies raised against the Mouse sequence would recognize Human Nestin.
• Formulation: 10mM PBS, pH 7.2 containing 0.02% Sodium Azide as preservative.
  State: Aff - Purified
  State: Liquid purified (filter sterilized) IgY fraction.
• Concentration: 0.3 mg/ml (based on Bradford assay readings using BSA as a standard).
• Purification: Affinity Chromatography using a peptide column.
• Gene Name: Mus musculus nestin (Nes)
• Database Link:
  Entrez Gene 25491 RatEntrez Gene 18008 Mouse
• Background: Mouse Nestin is a 206,994 dalton protein (1864 amino acids) expressed in neural crest cells, CNS neural stems cells, as well as a few non-neural cell types in the embryo, including cells within the pancreatic islets of Langerhans and the limb bud. Nestin is an intermediate filament-associated protein, and contributes to the cytoskeleton.
• Synonyms: NES, Nbla00170