NSE (ENO2) Chicken Polyclonal Antibody

CAT#: AP31816PU-N

NSE (ENO2) chicken polyclonal antibody, Aff - Purified

Product Images

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  Dissociated cell cultures of neonatal Mouse brains, showing NSE-2 (green staining) in neurons. These cultures were counter-stained with a Rabbit anti-GFAP antibody to localize astrocytes, as well as with DAPI (blue staining) to localize nuclei.

Specifications

Product Data

• Applications: IF, IHC, WB
• Recommended Dilution: Western Blot.
  Immunocytochemistry.
  Immunohistochemistry.
  Recommended Dilutions:
  1/5000-1/10000 for Western Blots.
  1/2000-1/5000 for Immunohistochemistry and Immunocytochemistry using 2% paraformaldehyde-fixed tissues or cells.
  Quality Control:Both of these anti-peptide antibodies were analyzed by Immunohistochemistry (at a dilution of 1/5000) using Fluorescein-labeled Goat anti-Chicken IgY (1/500 dilution, Cat.-No AP31795FC-N) as the secondary reagent.
• Reactivities: Human, Mouse, Rat
• Host: Chicken
• Isotype: Ig
• Clonality: Polyclonal
• Immunogen: Chickens were immunized with two synthetic peptide KLH conjugates corresponding to different regions of the NSE-2 gene product, but are shared between the Human (NP_001966, NCBI) and Rat (AAA41119) sequences.
  Production: After repeated injections into the hens, immune eggs were collected, and the IgY fractions were purified from the yolks. These IgY fractions were then affinity-purified using a peptide column, and the concentrations of the eluates adjusted to 0.2 mg/ml. Finally, equal volumes of each of these affinity-purified anti-peptide antibodies were mixed, and the preparation was filter-sterilized.
• Specificity: Recognizes Neuron-Specific Enolase-2 (NSE).
• Formulation: 10mM PBS, pH 7.2 containing 0.02% Sodium Azide as preservative.
  State: Aff - Purified
  State: Liquid purified (filter sterilized) IgY fraction.
• Concentration: lot specific
• Purification: Affinity Chromatography using a peptide column.
• Storage: Store the antibody undiluted in the dark at 2-8°C.
• Stability: Shelf life: one year from despatch.
• Gene Name: Homo sapiens enolase 2 (gamma, neuronal) (ENO2)
• Database Link:
  Entrez Gene 13807 MouseEntrez Gene 24334 RatEntrez Gene 2026 Human
• Background: Human NSE-2 (EC 4.2.1.11) is a 47,138 dalton protein (434 amino acids) expressed in neurons of the peripheral nervous system (PNS) and central nervous system (CNS).
  NSE-2 catalyzes the conversion of 2-phospho-D-glycerate into phosphoenol pyruvate, and is an essential enzyme in energy metabolism in nervous tissues.
• Synonyms: NSE, ENO2, Enolase 2,Neural enolase, Gamma-enolase

Reference Data

• Protein Pathways: Glycolysis / Gluconeogenesis, Metabolic pathways, RNA degradation