beta III Tubulin (TUBB3) Chicken Polyclonal Antibody

CAT#: AP31823PU-N

beta III Tubulin (TUBB3) chicken polyclonal antibody, Aff - Purified

Product Images

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  Dissociated cell culture of a neonatal Mouse brain showing Beta-Tubulin III (green staining) of neurons. These cultures were counter-stained with a Rabbit anti-GFAP antibody (red staining) to identify astrocytes, and with DAPI (blue staining) to localize nuclei.

Specifications

Product Data

• Applications: IF, IHC, WB
• Recommended Dilution: Western Blot.
  Immunocytochemistry.
  Immunohistochemistry.
  Recommended Dilutions:
  1/2000-1/5000 for Western Blots.
  1/1000-1/2000 for Immunohistochemistry and Immunocytochemistry using 2% paraformaldehyde-fixed tissues or cells.
  Quality Control: Each of the three antibodies was analyzed by Immunohistochemistry (at a dilution of 1/2000) using Fluorescein-labeled Goat anti-Chicken IgY (1/500 dilution, Cat.-No AP31795FC-N) as the secondary reagent.
• Reactivities: Human, Rat
• Host: Chicken
• Isotype: Ig
• Clonality: Polyclonal
• Immunogen: Chickens were immunized with three synthetic peptide KLH conjugated corresponding to different regions of the Beta-Tubulin 3 gene product, but are shared between the Human (AAL28094) and Rat (AAM28438) protein sequences.
  Production: After repeated injections, immune eggs were collected, and the IgY fractions were purified from the yolks. These IgY fractions were then affinity-purified using a peptide column, and the concentrations of the eluates adjusted to 0.3 mg/ml. Finally, equal volumes of each of the three affinity-purified anti-peptide antibodies were mixed, and the preparation was filter-sterilized.
• Specificity: Recognizes Tubulin beta-3.
• Formulation: 10mM PBS, pH 7.2 containing 0.02% Sodium Azide as preservative.
  State: Aff - Purified
  State: Liquid purified (filter sterilized) IgY fraction.
• Concentration: 0.3 mg/ml. The concentrations of each of the three antibodies was 0.1 mg/ml (based on Bradford assay readings using BSA as a standard).
• Purification: Affinity Chromatography.
• Gene Name: Homo sapiens tubulin beta 3 class III (TUBB3), transcript variant 2
• Database Link:
  Entrez Gene 246118 RatEntrez Gene 10381 Human
• Background: Human Beta-Tubulin 3 is a 50,432 dalton structural protein (450 amino acid) expressed in neurons of the PNS and CNS. It contributes to microtubule stability in neuronal cell bodies and axons, and plays a role in axonal transport.
• Synonyms: Tubulin beta-3 chain, Tubulin beta-III, Tubulin beta-4

Reference Data

• Protein Families: Druggable Genome, ES Cell Differentiation/IPS
• Protein Pathways: Gap junction, Pathogenic Escherichia coli infection