Spasmolytic Polypeptide (TFF2) Rabbit Polyclonal Antibody

CAT#: AP31836PU-N

Spasmolytic Polypeptide (TFF2) rabbit polyclonal antibody, Aff - Purified

Product Images

To detect Human TFF-2 by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 μg/ml. When used in conjunction with compatible secondary reagents the detection limit for recombinant Human TFF-2 is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.

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  To detect Human TFF-2 by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 μg/ml. When used in conjunction with compatible secondary reagents the detection limit for recombinant Human TFF-2 is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.

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  To detect Human TFF-2 by sandwich ELISA (using 100μl/well antibody solution) a concentration of 0.5 - 2.0 μg/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with Biotinylated Anti-Human TFF-2 as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant Human TFF-2.

Specifications

Product Data

• Applications: ELISA, WB
• Recommended Dilution: ELISA.
  Western Blot.
• Reactivities: Human
• Host: Rabbit
• Clonality: Polyclonal
• Immunogen: Highly pure E.coli derived Recombinant Human Trefoil Factor 2
• Specificity: Recognizes TFF-2
• Formulation: PBS, pH 7.2 without preservatives
  State: Aff - Purified
  State: Lyophilized purified Ig fraction
• Reconstitution Method: Restore in sterile water to a concentration of 0.1-1.0 mg/ml.
• Purification: Affinity Chromatography employing an immobilized Human TFF-2 matrix.
• Storage: Store the antibody prior to reconstitution at -20°C for one year.
  Following reconstitution the antibody can be stored at 2-8°C for 2 weeks.
  Avoid repeated freezing and thawing.
• Gene Name: Homo sapiens trefoil factor 2 (TFF2)
• Database Link:
  Entrez Gene 7032 Human
• Background: The Trefoil Factor peptides (TFF1, TFF2 and TFF3) are expressed in the gastrointestinal tract, and appear to play an important role in intestinal mucosal defense and repair. TFF2 has been shown to inhibit gastrointestinal motility and gastric acid secretion. Recent data suggests a potential role for TFF2 in acute and chronic asthma (Nikolaidis, N.M. et al. Am. Journal Respir. Cell Mol. Biol. (2003) 4: 458-464).
• Synonyms: TFF2, Spasmolytic polypeptide, SP, Spasmolysin
• Note: Centrifuge vial prior to opening!

Reference Data

• Protein Families: Druggable Genome, Secreted Protein