CTDSP1 Goat Polyclonal Antibody

CAT#: AP31837PU-N

CTDSP1 goat polyclonal antibody, Aff - Purified

Product Images

Staining of Mouse Testis lysate (35ug protein in RIPA buffer) using CTDSP1 Antibody at 0.1 ug/ml. Primary incubation was 1 hour. Detected by chemiluminescence.

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  Staining of paraffin embedded Human Tonsil using CTDSP1 Antibody at 5 ug/ml. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

Specifications

Product Data

• Applications: ELISA, IHC, WB
• Recommended Dilution: Peptide ELISA: 1/16000 (Detection Limit).
  Western blot: 0.1-0.3 µg/ml. Approx 30kDa band observed in Mouse Testis lysates (calculated MW of 29.3kDa according to Mouse NP_694728.1).
• Reactivities: Bovine, Canine, Human, Mouse, Rat
• Host: Goat
• Clonality: Polyclonal
• Immunogen: Peptide with sequence from the internal region of the protein sequence according to NP_067021.1; NP_872580.1.
• Specificity: This antibody is expected to recognise both reported isoforms (NP_067021 and NP_872580).
• Formulation: Tris buffered saline, pH~7.3 containing 0.02% Sodium Azide as preservative and 0.5% BSA as stabilizer
  State: Aff - Purified
  State: Liquid purified Ig fraction
• Concentration: 0.5 mg/ml
• Purification: Ammonium Sulphate Precipitation followed by antigen Affinity Chromatography using the immunizing peptide
• Gene Name: Homo sapiens CTD small phosphatase 1 (CTDSP1), transcript variant 3
• Database Link:
  Entrez Gene 227292 MouseEntrez Gene 363249 RatEntrez Gene 58190 Human
• Background: CTDSP1 is a class 2C phosphatase with activity dependent on the conserved DxD motif. Expression of CTDSP1 inhibited activated transcription from several promoter-reporter gene constructs, but expression of a mutant lacking phosphatase activity enhanced transcription. Neuronal gene transcription is repressed in nonneuronal cells by the repressor element-1 (RE1)-silencing transcription factor/neuron-restrictive silencer factor (REST/NRSF; 600571) complex. REST/NRSF recruits SCPs to neuronal genes that contain RE1 elements, leading to neuronal gene silencing in nonneuronal cells. Phosphatase-inactive forms of SCP interfere with REST/NRSF function and promote neuronal differentiation of P19 stem cells. Likewise, small interfering RNA directed to the single Drosophila SCP unmasks neuronal gene expression in S2 cells. Thus, SCP activity is an evolutionarily conserved transcriptional regulator that acts globally to silence neuronal genes.
• Synonyms: NIF3, NLIIF, SCP1, NLI-interacting factor 3, NLI-IF

Reference Data

• Protein Families: Phosphatase