MLX Goat Polyclonal Antibody

CAT#: AP31860PU-N

MLX goat polyclonal antibody, Aff - Purified

Product Images

Staining of paraffin embedded Human Placenta using MLX Antibody at 3.8 ug/ml. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

Specifications

Product Data

• Applications: ELISA, IHC
• Recommended Dilution: Peptide ELISA: 1/128000 (Detection Limit).
  Western blot: Preliminary experiments in Human, Mouse and Rat Liver lysates gave no specific signal but low background (at antibody concentration up to 1µg/ml). We would appreciate any feedback from people in the field - have any results been reported with other antibodies/lysates?
  Immunohistochemistry on Paraffin Sections: 2.5-3.8 µg/ml. In paraffin embedded Human Placenta shows nuclear stsaining of trophoblasts.
• Reactivities: Bovine, Canine, Human, Mouse, Rat
• Host: Goat
• Clonality: Polyclonal
• Immunogen: Peptide with sequence from the internal region of the protein sequence according to NP_937848.1; NP_937847.1; NP_733752.1.
• Specificity: This antibody is expected to recognize all three reported isoforms (NP_937848.1, NP_937847.1, NP_733752.1).
• Formulation: Tris buffered saline, pH~7.3 containing 0.02% Sodium Azide as preservative and 0.5% BSA as stabilizer
  State: Aff - Purified
  State: Liquid purified Ig fraction
• Concentration: lot specific
• Purification: Ammonium Sulphate Precipitation followed by antigen Affinity Chromatography using the immunizing peptide
• Storage: Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
  Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.
• Gene Name: Homo sapiens MLX, MAX dimerization protein (MLX), transcript variant 3
• Database Link:
  Entrez Gene 21428 MouseEntrez Gene 360631 RatEntrez Gene 6945 Human
• Background: MLX belongs to the family of basic helix-loop-helix leucine zipper (bHLH-Zip) transcription factors. These factors form heterodimers with Mad proteins and play a role in proliferation, determination and differentiation. This protein may act to diversify Mad family function by its restricted association with a subset of the Mad family of transcriptional repressors, namely, Mad1 and Mad4.
• Synonyms: MLX, TCFL4

Reference Data

• Protein Families: Druggable Genome, Transcription Factors