JNK2 (MAPK9) (217-230) Goat Polyclonal Antibody

CAT#: AP31925PU-N

JNK2 (MAPK9) (217-230) goat polyclonal antibody, Aff - Purified

Product Images

JNK2 antibody staining of HeLa lysate at 0.03 ug/ml (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence

Specifications

Product Data

• Applications: ELISA, WB
• Recommended Dilution:

  Peptide ELISA: 1/16000 (Detection limit).
  Western Blot: 0.03-0.1 µg/ml. Detects a band of ~48kDa band observed in lysates of cell line HeLa.

• Reactivities: Canine, Human, Mouse, Rat
• Host: Goat
• Clonality: Polyclonal
• Immunogen: Peptide with sequence from the internal region of the protein sequence according to NP_620707.1; NP_002743.3; NP_001128516.1.
• Specificity:
  This antibody is expected to recognize isoforms alpha 1, alpha 2. and gamma (NP_620707.1, NP_002743.3, NP_001128516.1).
• Formulation: Tris saline, pH~7.3
  State: Aff - Purified
  State: Liquid purified Ig fraction
  Stabilizer: 0.5% BSA
  Preservative: 0.02% Sodium Azide
• Concentration: lot specific
• Purification: Affinity Chromatography
• Storage:

  Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
  Avoid repeated freezing and thawing.

• Stability: Shelf life: one year from despatch.

• Predicted Protein Size: 48.1 kDa (NP_002743.3).
• Database Link:
  Entrez Gene 5601 Human
• Background: JNK2, a MAPK type protein kinase, regulates cell apoptosis, differentiation and growth in response to growth factors, inflammatory cytokines and cellular stress. JNK2 is phosphorylated by by MKK4/MAP2K4 and MKK7/MAP2K7. Activated JNK2 translocates to the nucleus where it phosphorylates transcription factors, including c-Jun, ATF2, ELK1, Jun-D and NFAT4. At least four distinct isoforms, resulting from alternatively spliced transcript variants, have been reported - three different isoforms comprised of 382 amino acids and one comprised of 424 amino acids.
• Synonyms: JNK-55, PRKM9, MAP kinase 9