Smgc Goat Polyclonal Antibody

CAT#: AP31966PU-N

Smgc goat polyclonal antibody, Aff - Purified

Product Images

AP31966PU-N (4 g/ml) staining of Mouse SubMandibular Gland cells at E18. Nuclear counterstain with DAPI in green.

Specifications

Product Data

• Applications: ELISA, IF
• Recommended Dilution:
  Peptide ELISA: 1/16000 (Detection limit).
  Western blot: Preliminary experiments gave bands at approx 65kDa and 70kDa in Mouse fetal Lung lysates after 1µg/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the bands we observe given the calculated size of 74.4kDa according to NP_945121.1. Both detected bands were successfully blocked by incubation with the immunizing peptide (and BLAST results with the immunizing peptide sequence did not identify any other proteins to explain the additional bands).
  Immunocytochemistry: 4 µg/ml. Positive staining of select epithelial cells in the murine Submanibular gland at E18. Data provided by Melinda Larsen, State University of New York, Albany, NY.
• Reactivities: Mouse
• Host: Goat
• Clonality: Polyclonal
• Immunogen: Synthetic peptide: KLEPKYENPTNGS with a Cysteine residue linker, corresponding to internal sequence amino acids 518-530 of Mouse Muc19 (NP_945121.1).
• Specificity: This antibody recognizes Mouse Muc19 /Smgc.
  Other species not tested.
• Formulation: Tris saline, pH~7.3
  State: Aff - Purified
  State: Liquid purified Ig fraction
  Stabilizer: 0.5% BSA
  Preservative: 0.02% Sodium Azide
• Concentration: 0.5 mg/ml
• Purification: Ammonium Sulphate Precipitation followed by Antigen Affinity Chromatography using the immunizing peptide
• Database Link:
  Entrez Gene 223809 Mouse
• Background: Detected in terminal tubule cells of the submandibular gland (at protein level). Expressed in submandibular salivary glands of 3-day-old males but not adults. Expression in adult submandibular glands is restricted to females. Isoform 5 is expressed in both 3-day-old and adult sublingual glands.
• Synonyms: Submandibular gland protein C, Muc19