NLRP3 Goat Polyclonal Antibody

CAT#: AP32694PU-N

NLRP3 goat polyclonal antibody, Aff - Purified

Product Images

(2 ug/ml) staining of paraffin embedded Human Tonsil. Steamed antigen retrieval with citrate buffer pH 6, HRP-staining.

Specifications

Product Data

• Applications: ELISA, IHC, WB
• Recommended Dilution: Peptide ELISA: 1/64000 (Detection Limit).
  Western blot: Preliminary experiments gave an approx 70kDa band in human lymph node and tonsil lysates after 0.3 µg/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 118kDa according to NP_004886.3 and 106kDa according to NP_899632.1. The 70kDa band was successfully blocked by incubation with the immunizing peptide.
  Immunohistochemistry on Paraffin Sections: 2-4 µg/ml. In paraffin embedded Human Tonsil shows focal and interstitial staining of the inflammatory elements close to the epithelia of the tonsil crypts.
• Reactivities: Bovine, Canine, Human, Mouse, Porcine, Rat
• Host: Goat
• Clonality: Polyclonal
• Immunogen: Peptide with sequence from the internal region of the protein sequence according to NP_004886.3; NP_899632.1; NP_001230062.1; NP_001120933.1; NP_001120934.1.
• Specificity: This antibody is expected to recognise all five reported isoforms (NP_004886.3; NP_899632.1; NP_001230062.1; NP_001120933.1; NP_001120934.1).
  Reported variants represent identical protein (NP_004886.3 and NP_001073289.1).
• Formulation: Tris saline, pH~7.3
  State: Aff - Purified
  State: Liquid purified IgG fraction
  Stabilizer: 0.5% BSA
  Preservative: 0.02% Sodium Azide
• Concentration: 0.5 mg/ml
• Purification: Ammonium Sulphate Precipitation followed by Antigen Affinity Chromatography using the immunizing peptide
• Database Link:
  Entrez Gene 114548 Human
• Background: NLRP3 is a pyrin-like protein containing a pyrin domain, a nucleotide-binding site (NBS) domain, and a leucine-rich repeat (LRR) motif. This protein interacts with the apoptosis-associated speck-like protein PYCARD/ASC, which contains a caspase recruitment domain, and is a member of the NALP3 inflammasome complex. This complex functions as an upstream activator of NF-kappaB signaling, and it plays a role in the regulation of inflammation, the immune response, and apoptosis.
• Synonyms: NLRP3, C1orf7, CIAS1, PYPAF1