NLRP3 Goat Polyclonal Antibody

CAT#: AP32694PU-N

NLRP3 goat polyclonal antibody, Aff - Purified


USD 375.00

5 Days*

Size
    • 100 ug

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Specifications

Product Data
Applications ELISA, IHC, WB
Recommended Dilution Peptide ELISA: 1/64000 (Detection Limit). 
Western blot: Preliminary experiments gave an approx 70kDa band in human lymph node and tonsil lysates after 0.3 µg/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 118kDa according to NP_004886.3 and 106kDa according to NP_899632.1. The 70kDa band was successfully blocked by incubation with the immunizing peptide. 
Immunohistochemistry on Paraffin Sections: 2-4 µg/ml. In paraffin embedded Human Tonsil shows focal and interstitial staining of the inflammatory elements close to the epithelia of the tonsil crypts.
Reactivities Bovine, Canine, Human, Mouse, Porcine, Rat
Host Goat
Clonality Polyclonal
Immunogen Peptide with sequence from the internal region of the protein sequence according to NP_004886.3; NP_899632.1; NP_001230062.1; NP_001120933.1; NP_001120934.1.
Specificity This antibody is expected to recognise all five reported isoforms (NP_004886.3; NP_899632.1; NP_001230062.1; NP_001120933.1; NP_001120934.1).
Reported variants represent identical protein (NP_004886.3 and NP_001073289.1).
Formulation Tris saline, pH~7.3
State: Aff - Purified
State: Liquid purified IgG fraction
Stabilizer: 0.5% BSA
Preservative: 0.02% Sodium Azide
Concentration 0.5 mg/ml
Purification Ammonium Sulphate Precipitation followed by Antigen Affinity Chromatography using the immunizing peptide
Background NLRP3 is a pyrin-like protein containing a pyrin domain, a nucleotide-binding site (NBS) domain, and a leucine-rich repeat (LRR) motif. This protein interacts with the apoptosis-associated speck-like protein PYCARD/ASC, which contains a caspase recruitment domain, and is a member of the NALP3 inflammasome complex. This complex functions as an upstream activator of NF-kappaB signaling, and it plays a role in the regulation of inflammation, the immune response, and apoptosis.
Synonyms NLRP3, C1orf7, CIAS1, PYPAF1
Reference Data

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