PRAK (MAPKAPK5) Goat Polyclonal Antibody

CAT#: AP32808PU-N

PRAK (MAPKAPK5) goat polyclonal antibody, Aff - Purified

Product Images

HEK293 lysate (10 ug protein in RIPA buffer) overexpressing Mouse MK5-GFP (first lane) or Mouse MK2-GFP (second lane) probed with (0.5 ug/ml) in right panel and with -N (0.5 ug/ml) on left panel, Primary incubations were for 2 hours. Detected by chemiluminescence.

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  (5 ug/ml) staining of paraffin embedded Human Kidney. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

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  and (1.5 ug) immunoprecipitations from lysates of MK5/ERK3 double knockout MEFs, with (third and fifth lanes) and without (fourth and sixth lanes) rescued MK5/ERK3 expression through retroviral transduction. The corresponding lysates (first and second lane resp.) were analyzed in parallel in this Western blot labelled with mouse anti-MK5 / PRAK (and co-precipitation was measured using Rabbit anti-ERK3 in the lower panel).

Specifications

Product Data

• Applications: ELISA, IHC, IP, WB
• Recommended Dilution: Peptide ELISA: 1/32000 (Detection Limit).
  Western blot: 0.5-1 μg/ml. In transfected HEK293 transiently expressing either Mouse MK5 fused to GFP or the similar Mouse MK2 fused to GFP; only the expected band was observed in the MK5 lane after AP32808PU-N labeling.
  Data obtained from Dr. M. B. Menon, Inst. Biochemistry, Hannover Medical School, Germany.
  Immunoprecipitation: MK5/ERK3 double knockout Mouse Embryonic Fibroblasts (MEFs) retrovirally transduced wiith Mk5/Erk3 or empty vector (GFP) were lysed from confluent plates and used for IP with 1.5 µg AP32808PU-N or AP32809PU-N.
  Western blots of the Immunoprecipitation were labelled with Mouse anti-MK5 (SC) or with Rabbit anti-ERK3 serum (CST). An approx 55kDa of MK5 is only precipitated from lysates of those KO MEFs that have been rescued by the ERK3/MK5 expression constructas described in PMID: 22508986.
  Data obtained from Dr. M. B. Menon, Inst. Biochemistry, Hannover Medical School, Germany.
  Immunohistochemistry on Paraffin Sections: 4-8 µg/ml. In paraffin embedded Human Kidney shows brushborder staining in the PCT.
• Reactivities: Bovine, Canine, Human, Mouse, Porcine, Rat
• Host: Goat
• Clonality: Polyclonal
• Immunogen: Peptide with sequence from the internal region of the protein sequence according to NP_003659.2; NP_620777.1.
• Specificity: Recognizes MK5 / MAPKAPK5.
• Formulation: Tris saline, pH~7.3
  State: Aff - Purified
  State: Liquid purified Ig fraction
  Stabilizer: 0.5% BSA
  Preservative: 0.02% Sodium Azide
• Concentration: 0.5 mg/ml
• Purification: Ammonium Sulphate Precipitation followed by antigen Affinity Chromatography using the immunizing peptide
• Database Link:
  Entrez Gene 8550 Human
• Synonyms: MAPK-activated protein kinase 5, MAPKAP kinase 5, MAPKAPK5, PRAK