PRAK (MAPKAPK5) Goat Polyclonal Antibody
Specifications
Product Data | |
Applications | ELISA, IHC, IP, WB |
Recommended Dilution | Peptide ELISA: 1/32000 (Detection Limit). Western blot: 0.5-1 μg/ml. In transfected HEK293 transiently expressing either Mouse MK5 fused to GFP or the similar Mouse MK2 fused to GFP; only the expected band was observed in the MK5 lane after AP32808PU-N labeling. Data obtained from Dr. M. B. Menon, Inst. Biochemistry, Hannover Medical School, Germany. Immunoprecipitation: MK5/ERK3 double knockout Mouse Embryonic Fibroblasts (MEFs) retrovirally transduced wiith Mk5/Erk3 or empty vector (GFP) were lysed from confluent plates and used for IP with 1.5 µg AP32808PU-N or AP32809PU-N. Western blots of the Immunoprecipitation were labelled with Mouse anti-MK5 (SC) or with Rabbit anti-ERK3 serum (CST). An approx 55kDa of MK5 is only precipitated from lysates of those KO MEFs that have been rescued by the ERK3/MK5 expression constructas described in PMID: 22508986. Data obtained from Dr. M. B. Menon, Inst. Biochemistry, Hannover Medical School, Germany. Immunohistochemistry on Paraffin Sections: 4-8 µg/ml. In paraffin embedded Human Kidney shows brushborder staining in the PCT. |
Reactivities | Bovine, Canine, Human, Mouse, Porcine, Rat |
Host | Goat |
Clonality | Polyclonal |
Immunogen | Peptide with sequence from the internal region of the protein sequence according to NP_003659.2; NP_620777.1. |
Specificity | Recognizes MK5 / MAPKAPK5. |
Formulation | Tris saline, pH~7.3 State: Aff - Purified State: Liquid purified Ig fraction Stabilizer: 0.5% BSA Preservative: 0.02% Sodium Azide |
Concentration | 0.5 mg/ml |
Purification | Ammonium Sulphate Precipitation followed by antigen Affinity Chromatography using the immunizing peptide |
Database Link | |
Synonyms | MAPK-activated protein kinase 5, MAPKAP kinase 5, MAPKAPK5, PRAK |
Reference Data |
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