CD19 (422-433) Goat Polyclonal Antibody

CAT#: AP32823PU-N

CD19 (422-433) goat polyclonal antibody, Aff - Purified

Product Images

Staining of Jurkat lysate using CD19 Antibody at 2 ug/ml (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Specifications

Product Data

• Applications: ELISA, WB
• Recommended Dilution: Peptide ELISA: 1/32000 (Detection Limit).
  Western blot: 1-3 µg/ml. Approx 75kDa band observed in lysates of cell lines Jurkat and MOLT4 (calculated MW of 61.1kDa according to NP_NP_001761.3). The observed molecular weight corresponds to the glycosylated form.
• Reactivities: Bovine, Human
• Host: Goat
• Clonality: Polyclonal
• Immunogen: Peptide with sequence from the internal regionm (422-433) of the protein sequence according to NP_001171569.1; NP_001761.3.
• Specificity: This antibody is expected to recognize both reported isoforms (NP_001171569.1; NP_001761.3).
• Formulation: Tris saline, pH~7.3
  State: Aff - Purified
  State: Liquid purified Ig fraction
  Stabilizer: 0.5% BSA
  Preservative: 0.02% Sodium Azide
• Concentration: lot specific
• Purification: Ammonium Sulphate Precipitation followed by antigen Affinity Chromatography using the immunizing peptide
• Storage: Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
  Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.
• Database Link:
  Entrez Gene 930 Human
• Synonyms: Leu-12, B-cell marker