LYZ Rabbit Polyclonal Antibody

CAT#: AP32827AF-N

LYZ rabbit polyclonal antibody, Azide Free

Product Images

Formalin-Fixed, Paraffin-Embedded Human Spleen stained with Lysozyme C Antibody at 1/400 dilution after heat-induced antigen retrieval.

Specifications

Product Data

• Applications: ELISA, IF, IHC, WB
• Recommended Dilution: The cytochemical grade allows the use in different types of highly sensitive immunoassays on appropriately treated cell and tissue substrates; in radioimmunoassay; for the production of immunoconjugates with a selected marker; to prepare immunoaffinity adsorbents by coupling to an artificial carrier; in non-isotopic methodology based on solid phase immunochemistry (e.g. ELISA), both as catching antibody and detection reagent; in Western blotting. This product is not pre-diluted.
  Recommended Working Dilutions:
  Histochemical and cytochemical: 1/100-1/250.
  ELISA and comparable non-precipitating antibody-binding assays: 1/500-1/5,000.
  Immunohistochemistry on Paraffin Sections: 1/400.
• Reactivities: Human
• Host: Rabbit
• Isotype: IgG
• Clonality: Polyclonal
• Immunogen: Highly purified lysozyme is isolated from pooled milk. Freund's complete adjuvant is used in the first step of the immunization procedure.
• Specificity: The reactivity of the antiserum is restricted to lysozyme. In immunoelectrophoresis and radial immunodiffusion (Ouchterlony), using various antiserum concentrations against normal human milk and tears a single precipitin line is obtained which shows a reaction of identity with the precipitin line obtained with purified lysozyme. No reaction is obtained with any other plasma protein component or serum.
  Cross-reactivity: The antiserum does not cross-react with any other component of human plasma.
  Inter-species cross-reactivity is a normal feature of antibodies to plasma proteins since they frequently share antigenic determinants. Cross-reactivity of this antiserum has not been tested in detail.
• Formulation: PBS, pH 7.2
  State: Azide Free
  State: Lyophilized Purified hyperimmune IgG fraction
  Stabilizer: None
  Preservative: None
• Reconstitution Method: Restore by by adding 1 ml sterile di stilled water
• Concentration: 10.0 mg/ml
• Purification: Hyperimmune antisera with strong precipitating activity are selected for fractionation by salt precipitation and purification of the IgG fraction by DEAE-chromatography
• Database Link:
  Entrez Gene 396218 Chicken
• Background: Lysozyme, a bacteriolytic enzyme, has a molecular weight of about 15,000. Its concentration in plasma is low; in milk the concentration is reported to be between 5 and 220 mg/ml. Also tears contain a considerable amount of lysozyme.
• Synonyms: LYZ, LZM
• Note: Adsorption: Immunoaffinity adsorbed using insolubilized antigens as required, to eliminate antibodies cross-reacting with other with other plasma proteins. The use of insolubilized adsorption antigens prevents the presence of excess adsorbent protein or immune complexes in the antiserum.