Doublecortin (DCX) Chicken Polyclonal Antibody

CAT#: AP32839PU-N

Doublecortin (DCX) chicken polyclonal antibody, Purified

Product Images

Doublecortin staining of cochlear neurons in the cochlear ganglion of an adult Mouse brain (green), reed staining is neurofilament immunoreactivity, as seen using Rabbit antibody.

Specifications

Product Data

• Applications: IF, IHC
• Recommended Dilution: Immunofluorescence/Immnunocytochemistry.
  Immunohistochemistry.
  Quality Control: This anti-peptide antibody mixture was validated by Immunohistochemistry (at a concentration of 2 µg/ml) using Fluorescein Conjugated Affinity-purified Goat anti-Chicken IgY antibody (Cat.-No AP31795FCN, 1/5000 dilution) as a secondary antibody.
  Recommended Dilutions: 1/1000-1/2000 for Immunohistochemistry and Immunocytochemistry using 2% paraformaldehyde-fixed tissues or cells.
• Reactivities: Human, Mouse
• Host: Chicken
• Isotype: Ig
• Clonality: Polyclonal
• Immunogen: Chickens were immunized with two synthetic peptide / keyhole limpet hemocyanin (KLH) conjugates.
  Production: These synthetic peptides corresponded to different regions of the Doublecortin gene product, but are shared between the Human (CAA06617.1, NCBI) and Mouse (AAT58219.1, NCBI) sequences. After repeated injections, immune eggs were collected, and the IgY fractions were purified from the yolks. These IgY fractions were then affinity-purified using a peptide column, and the concentrations of the eluates adjusted to 0.2 mg/ml. Finally, equal volumes of these affinity-purified anti-peptide antibodies were mixed, and the preparation was filter-sterilized.
• Formulation: Sodium Phosphate-Buffered (10 mM) isotonic (0.9%, w/v) Saline (PBS, pH 7.2)
  State: Purified
  State: Liquid purified IgY fraction
  Preservative: 0.02% Sodium Azide
• Concentration: lot specific
• Purification: Affinity Chromatography
• Storage: Store undiluted at 2-8°C.
  This product is photosensitive and should be protected from light.
• Stability: Shelf life: one year from despatch.
• Database Link:
  Entrez Gene 1641 Human
• Synonyms: Lissencephalin-X, Doublin, DCX, DBCN, LISX