Actin Chicken Polyclonal Antibody

CAT#: AP32845PU-N

Actin chicken polyclonal antibody, Aff - Purified

Specifications

Product Data

• Applications: WB
• Recommended Dilution: Quality Control: This anti-Actin preparation was validated by Western blotting on nitrocellulose membranes (at a concentration of 1 µg/ml) using HRP-Conjugated Goat anti-Chicken IgY (1/5000 dilution, Cat.-No AP31795HR-N) as the secondary reagent.
  Recommended Dilutions: 1/1000-1/2000 for Western blot analysis of Nitrocellulose membranes.
  Please note that these dilutions are meant to serve as starting points, and that optimal dilutions may vary.
  Do Not use this product with PVDF, or other nylon-based membranes, because this often produces unacceptably high backgrounds in chemiluminescence studies.
• Reactivities: Rabbit
• Host: Chicken
• Isotype: Ig
• Clonality: Polyclonal
• Immunogen: Chickens were immunized with actin purified from Rabbit muscle.
  After epeated injections, immune eggs were collected, and the IgY fractions were purified from the yolks. These IgY fractions were then affinity-purified using a peptide column. The final product contains 50% glycerol (v/v), purified anti-Actin IgY fraction (10 mg/ml) spiked with 25 µg/ml of affinity purified anti-Actin IgY, and then filter-sterilized.
• Formulation: Phosphate-buffered (10 mM) isotonic (0.9%, w/v) saline (PBS, pH 7.2)
  State: Aff - Purified
  State: Liquid purified IgY fraction
  Preservative: 0.02% (w/v) Sodium Azide
• Concentration: 10 mg/ml of purified anti-Actin (purified from rabbit muscle) IgY spiked with 25 ug/ml of affinity purified anti-Actin IgY (based on A280 nm assay readings)
• Purification: Affinity Chromatography