TAPA1 (CD81) Rat Monoclonal Antibody [Clone ID: QV-6A8-S3]

CAT#: AP32979PU-N

TAPA1 (CD81) rat monoclonal antibody, clone QV-6A8-S3, Purified

Product Images

SDS-PAGE analysis of purified QV-6A8-S3 monoclonal antibody. Lane 1: molecular weight marker, Lane 2: 2 ug of purified QV-6A8-S3 antibody. Proteins were separated by SDSPAGE and stained with RAPID StainTM Reagent.

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  FACS analysis of Dubca cells using QV-6A8-S3. Dubca cells were transiently transfected with an expression vector encoding CD81 (red curve). Binding of QV-6A8-S3 was detected with a PE-conjugated secondary antibody. (Isotype control: black curve)

Specifications

Product Data

• Clone Name: QV-6A8-S3
• Applications: ELISA, FC, WB
• Recommended Dilution: Flow Cytometry: 1.2 µg/10⁶ cells.
  ELISA: 1/200-1/400.
  Cell based ELISA with intakt, transiently transfected cells: 1/200-1/400.
  
• Reactivities: Human
• Host: Rat
• Isotype: IgG2b
• Clonality: Monoclonal
• Immunogen: cDNA encoding Human CD81
• Specificity: Recognizes Human CD81.
  Other species not tested.
  Selection: Based on recognition of the complete native protein expressed on transfected mammalian cells.
• Formulation: PBS, pH 7.2
  State: Purified
  State: Liquid purified IgG fraction
• Concentration: lot specific
• Purification: Affinity Chromatography on Protein G
• Storage: Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
  Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.
• Database Link:
  Entrez Gene 975 Human
• Background: CD81 (TAPA-1) belongs to the transmembrane 4 superfamily (tetraspanin family). CD81 is a widely expressed cell-surface protein that is characterized by the presence of four transmembranic domains, short N and C termini, a small extracellular loop (SEL) and a large extra-cellular loop (LEL) (1). It is expressed on cells of hematopoietic, neuroectodermal and mesenchymal origin and plays a role in the regulation of cell development, cell-growth and signal transduction. CD81 plays a critical role in Hepatitis C Infection and is involved in HCV entry due to its ability to interact with virus' E1/E2 glycoproteins (2,3). CD81 is an essential HCV host factor as silencing of CD81 expression by CD81-specific monoclonal antibodies in hepatoma cells inhibits HCV entry (4).
• Synonyms: S5.7; TAPA-1; TAPA1; Tetraspanin-28; Tspan-28; TSPAN28