Adipophilin / ADFP (C-term) Guinea Pig Polyclonal Antibody

CAT#: AP33138SU-N

Adipophilin / ADFP (C-term) guinea pig polyclonal antibody, Serum

Specifications

Product Data

• Applications: IF, IHC, WB
• Recommended Dilution: Immunoblotting (Western blot): 1/2000.
  Immunohistochemistry on Frozen Sections: 1/100. For staining protocols see ref. Ohsaki et al.
  Immunofluorescence: 1/100.
  Cytological Material.
  Incubation Time: 1 h at room temperature or overnight at 2-8°C.
• Reactivities: Mouse
• Host: Guinea Pig
• Clonality: Polyclonal
• Immunogen: Synthetic peptide (C-terminal aa 413-425 of Mouse Adipophilin).
• Specificity: Specific for Adipophilin / ADRP / PLIN2, MW 46,646 (calculated from aa sequence data); apparent Mr 50,000 (after SDSPAGE); pI 6.42.
  Tissue Immunolocalization: Adipophilin / PLIN2 is positively detected in the glandular cells of lactating mammary gland (ductal cells are negative), zona fasciculata of the adrenal gland, Sertoli cells of the testis, and in fat-accumulating hepatocytes; adipocytes are negative.
  Tested Reactivities on Cultured Cell Lines: 3T3-L1 (mouse preadipocytes), OP9 (derived from mouse bone marrow), HL-1 (derived from AT-1 mouse cardiomyocyte tumor lineage).
  Negative Species: Human.
• Formulation: State: Serum
  State: Liquid Stabilized Antiserum
  Preservative: 0.09% Sodium Azide
• Background: Adipophilin / ADRP (a member of the PAT family) is a ubiquitous component of lipid droplets. It has been found in milk fat globule membranes and on the surface of lipid droplets in various cultured cell lines (see e.g. Heid et al.; for review see e.g. Targett-Adams et al.); inducible by etomoxir. Enhanced expression of Adipophilin / ADRP is a useful marker for pathologies characterized by increased lipid droplet accumulation. Such diseases include atheroma, steatosis, obesity and certain cases of liposarcoma. It also seems to be a potent marker for atherosclerosis. ADRP can also be used to study the virus entry of e.g. HCV via lipid droplets (see e.g. Hope et al.).
• Synonyms: Adipose differentiation-related protein, ADRP