Vimentin (VIM) (N-term) Guinea Pig Polyclonal Antibody

CAT#: AP33206SU-N

Vimentin (VIM) (N-term) guinea pig polyclonal antibody, Serum

Specifications

Product Data

• Applications: IHC, WB
• Recommended Dilution: Immunoblotting (Western blot): 1/2000.
  Immunohistochemistry on Frozen Sections: 1/100.
  Immunohistochemistry on Paraffin Sections: 1/50 (after microwave treatment).
  Cytological Material.
  Incubation Time: 1 h at RT extended with paraffin sections.
• Reactivities: Human, Mouse
• Host: Guinea Pig
• Clonality: Polyclonal
• Immunogen: Synthetic N-terminus of Human Vimentin (2–17).
• Specificity: Specific detection of Vimentin (Mr 57,000 polypeptide) in cells and tissues of mesenchymal origin (e.g. fibroblasts, endothelial cells, smooth muscle cells).
  Tumors Specifically Detected: Sarcoma (including myosarcoma), lymphoma, melanoma.
• Formulation: State: Serum
  State: Liquid Stabilized Antiserum
  Preservative: 0.09% Sodium Azide
• Storage: Store undiluted at 2-8°C.
• Stability: Shelf life: one year from despatch.
• Database Link:
  Entrez Gene 7431 Human
• Background: Vimentin is the main intermediate filament protein in mesenchymal cells. This protein is believed to be involved with the intracellular transport of proteins between the nucleus and plasma membrane. Vimentin is expressed in sarcomas of neural, muscle and fibroblast origin, but carcinomas are generally negative. Melanomas, lymphomas and vascular tumors may all stain for Vimentin.
• Synonyms: VIM