TNF alpha (TNF) Goat Polyclonal Antibody

CAT#: AP33336PU-S

TNF alpha (TNF) goat polyclonal antibody, Purified


USD 260.00

2 Weeks*

Size
    • 50 ug

Product Images

Specifications

Product Data
Applications ELISA, IHC, WB
Recommended Dilution Sandwich ELISA: To detect Human TNF-alpha by Sandwich ELISA (using 100 μl/well antibody solution) a concentration of 0.5-2.0 μg/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with Biotinylated Anti-Human TNF-alpha (Cat.-No AP33336BT) as a detection antibody, allows the detection of at least 0.2-0.4 ng/well of recombinant Human TNF-alpha.
Western Blot: To detect Human TNF-α by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 µg/ml. When used in conjunction with compatible secondary reagents, the detection limit for recombinant Human TNF-alpha is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions. 
Neutralization: To yield one-half maximal inhibition [ND50] of the biological activity of Human TNF-α (0.25 ng/ml), a concentration of 0.012-0.014 µg/ml of this antibody is required. 
Immunohistochemistry on Parrafin Sections: This antibody stained formalin-fixed, paraffin-embedded sections of human breast invasive ductal carcinoma. The recommended concentrations are 2.5 μg/ml-5.0 μg/ml with a two-hour incubation at room temperature or an overnight incubation at 4°C. An HRP-labeled polymer detection system was used with a DAB chromogen. This antibody was also tested in an HRP-biotin labeling detection system. Heat induced antigen retrieval with a pH 6.0 Sodium Citrate buffer is recommended. Optimal concentrations and conditions may vary.
Reactivities Human
Host Goat
Clonality Polyclonal
Immunogen Highly pure (> 98%) E.coli derived recombinant Human TNF-alpha (Cat.-No PA132)
Specificity This antibody recognizes Human TNF-alpha. Other species not tested.
Formulation PBS, pH 7.2
State: Purified
State: Lyophilized purified Ig fraction
Reconstitution Method Restore in sterile PBS containing 0.1% BSA to a concentration of 0.1-1.0 mg/ml.
Purification Affinity Chromatography employing immobilized Human TNF-alpha matrix.
Storage Store lyophilized at 2-8°C for 6 months or at -20°C long term.
After reconstitution store the antibody undiluted at 2-8°C for one month 
or (in aliquots) at -20°C long term.
Avoid repeated freezing and thawing.
Stability Shelf life: one year from despatch.
Gene Name tumor necrosis factor
Synonyms TNF, TNF-a, TNFA, TNFSF2, Cachectin
Reference Data

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*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.