GDNF Receptor alpha 1 (GFRA1) Chicken Polyclonal Antibody

CAT#: AP33455PU-N

GDNF Receptor alpha 1 (GFRA1) chicken polyclonal antibody, Aff - Purified

Product Images

Western-Blot detection of human GFRa-1, using Chicken polyclonal antibody to human GFRa-1. GFRa-1 was C-terminally His tagged. Protein was expressed by CHO cell culture. 100 ng of purified protein was loaded per lane. Lane 1. Prestained protein ladder (Naxo 8003). Liae 2. hGFRa-1. Primary antibody dilution 1:5000 was used. Rabbit anti-Chicken antibody was used as secondary antibody.

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  Immunofluorescence detection of human GFRa-1 expressed in U2OS cells. GFRa-1 was visualized using Chicken polyclonal antibody to human GFRa-1 (dilution 1:2000) and Goat anti-chicken IgY - H&L DyLight 550 (dilution 1:200) as secondary antibody. For nuclear staining DAPI was used. ArrayScan VTI platform (Thermo Scientific) was used for image acquisition (10x objective). Composite picture was generated using pseudocolors green for GFRa-1 specific signal and blue for nuclei.

Specifications

Product Data

• Applications: ELISA, IF, WB
• Recommended Dilution: ELISA: 1/80000-1/160000.
  Western blot: 1/5000-1/10000.
  Immunofluorescence: 1/1000-1/2000.
• Reactivities: Human
• Host: Chicken
• Isotype: IgY
• Clonality: Polyclonal
• Immunogen: Recombinant His-tagged human GFRa-1 protein produced using CHO-based.
  For production of hGFRa-1, glycosylphosphatidyl-inositol GPI-anchor was removed and protein was secreted to the cell culture supernatant.
  Protein was purified by Ni-affinity chromatography following gel-filtration from cell culture supernatant.
• Specificity: This antibody detects Human GFRa-1 and doesn’t cross-react with hGFRa-2, hGFRa-3, hGFRa-4.
  Other species not tested.
• Formulation: Concentrated Ammonium Sulphate in PBS pH 7.4
  State: Aff - Purified
  State: Liquid purified IgY fraction
• Concentration: lot specific
• Purification: Affinity Chromatography
• Storage: Upon receipt, store at 2-8°C.
  As product is (NH4)₂SO₄ precipitate, mix well by pipetting or vortexing prior use.
• Stability: Shelf life: One year from despatch.
• Database Link:
  Entrez Gene 2674 Human
• Background: Glial cell line-derived neurotrophic factor (GDNF) and neurturin (NTN) are two structurally related, potent neurotrophic factors that play key roles in the control of neuron survival and differentiation. The GFRa-1 is a member of the GDNF receptor family. It is a glycosylphosphatidylinositol (GPI)-linked cell surface receptor for both GDNF and NTN, and mediates activation of the RET tyrosine kinase receptor (www.ncbi.nlm.nih.gov/gene/2674).
• Synonyms: GFR-alpha-1, GDNF receptor alpha, RET ligand 1, GDNFRA, RETL1, TRNR1