Synaptopodin (SYNPO) (N-term) Guinea Pig Polyclonal Antibody

CAT#: AP33487SU-N

Synaptopodin (SYNPO) (N-term) guinea pig polyclonal antibody, Serum

Product Images

Synaptopodin staining in human glomeruli (AP33487PU-; dilution 1:50; Image courtesy of A.Weins, Brigham and Women's Hospital)

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  Synaptopodin staining in human glomeruli (AP33487PU-N; dilution 1:100; Image courtesy of A.Weins, Brigham and Women's Hospital).

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  Synaptopodin immunofluorescence staining in human podocytes. (Image courtesy of A.Weins, Brigham and Women's Hospital).

Specifications

Product Data

• Applications: IF, IHC, WB
• Recommended Dilution: Immunoblotting (Western): 1/500-1/1000.
  Immunofluorescence.
  Immunohistochemistry on Frozen Sections: 1/50-1/100.
  Immunohistochemistry on Paraffin Sections: 1/50-1/100 (after microwave treatment).
  Incubation Time:1 h at RT for immunohistochemistry.
• Reactivities: Human, Mouse
• Host: Guinea Pig
• Clonality: Polyclonal
• Immunogen: Synthetic peptides (Mouse N-terminus), coupled to KLH
• Specificity: The antibody reacts specifically with Synaptopodin, a prolin-rich actin-binding protein with 2 binding sites for actin. Synaptopodin belongs to actin-binding proteins, it has first been localized in podocytes and a subset of telencephalic postsynaptic densities. In human tissue synaptopodin has a molecular weight of 73.7 kD and pI of 9.38 (calculated from sequence data); in mouse the cor-responding data are 74 kD, pI 9.27. In SDS-PAGE the antigen appears as 100 kD polypeptide in brain and 110 kD polypeptide in kidney (attributed to post-translational modifications). In Western blot analysis the antibody also reacts with a 44 kD degradation fragment of synaptopodin.
  The antibody recognizes differentiated podocytes (glomerular visceral epi-thelial cells) in vivo and in vitro (weaker additional reaction with arterial endo-thelial cells), co-localization with alpha-actinin.
  Reacts with a subset of exclusively telencephalic synapses. Differentiation-dependent expression during postnatal maturation of murine brain. Differentia-tion-dependent expression in cultured hippocampal neurons.
• Formulation: State: Serum
  State: Liquid Stabilized Antiserum
  Preservative: 0.09% Sodium Azide
• Database Link:
  Entrez Gene 11346 Human
• Synonyms: KIAA1029