Calreticulin (CALR) Rabbit Polyclonal Antibody

CAT#: AP33519PU-N

Calreticulin (CALR) rabbit polyclonal antibody, Aff - Purified

Product Images

CALR Antibody (Cat.-No AP33519PU-N) immunohistochemistry analysis in formalin fixed and paraffin embedded human colon carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the CALR Antibody for immunohistochemistry. Clinical relevance has not been evaluated.

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  CALR Antibody (Cat.-No AP33519PU-N) western blot analysis in Hela,SH-SY5Y,rat C6 cell line lysates (35ug/lane).This demonstrates the CALR antibody detected the CALR protein (arrow).antibody detected the CALR protein (arrow).

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  All lanes: Anti-CALR Antibody at 1:1000 dilution. Lane 1: Hela whole cell lysate. Lane 2: SH-SY5Y whole cell lysate Lysates/proteins at 20 μg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 48 kDa Blocking/Dilution buffer: 5% NFDM/TBST.Blocking/Dilution buffer: 5% NFDM/TBST.

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  CALR Antibody (Cat.-No AP33519PU-N) immunohistochemistry analysis in formalin fixed and paraffin embedded human colon carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the CALR Antibody for immunohistochemistry. Clinical relevance has not been evaluated.

Specifications

Product Data

• Applications: ELISA, FC, IHC, WB
• Recommended Dilution: Western blot: 1/100-1/500.
  ELISA: 1/1,000.
  Flow Cytometry: 1/10-1/50.
  Immunohistochemistry on Paraffin Sections: 1/10-1/50.
  
• Reactivities: Human, Rat
• Host: Rabbit
• Clonality: Polyclonal
• Immunogen: This CALR Antibody is generated from rabbits immunized with a recombinant protein of human CALR.
• Specificity: This antibody detects Calreticulin.
• Formulation: PBS with 0.09% (W/V) sodium azide
  State: Aff - Purified
  State: Liquid Ig fraction
• Concentration: lot specific
• Purification: Protein A column, followed by peptide affinity purification
• Storage: Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
  Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.
• Gene Name: Homo sapiens calreticulin (CALR)
• Database Link:
  Entrez Gene 64202 RatEntrez Gene 811 Human
• Background: Calreticulin is a multifunctional protein that acts as a major Ca(2+)-binding (storage) protein in the lumen of the endoplasmic reticulum. It is also found in the nucleus, suggesting that it may have a role in transcription regulation. Calreticulin binds to the synthetic peptide KLGFFKR, which is almost identical to an amino acid sequence in the DNA-binding domain of the superfamily of nuclear receptors. Calreticulin binds to antibodies in certain sera of systemic lupus and Sjogren patients which contain anti-Ro/SSA antibodies, it is highly conserved among species, and it is located in the endoplasmic and sarcoplasmic reticulum where it may bind calcium. The amino terminus of calreticulin interacts with the DNA-binding domain of the glucocorticoid receptor and prevents the receptor from binding to its specific glucocorticoid response element. Calreticulin can inhibit the binding of androgen receptor to its hormone-responsive DNA element and can inhibit androgen receptor and retinoic acid receptor transcriptional activities in vivo, as well as retinoic acid-induced neuronal differentiation. Thus, calreticulin can act as an important modulator of the regulation of gene transcription by nuclear hormone receptors. Systemic lupus erythematosus is associated with increased autoantibody titers against calreticulin but calreticulin is not a Ro/SS-A antigen. Earlier papers referred to calreticulin as an Ro/SS-A antigen but this was later disproven. Increased autoantibody titer against human calreticulin is found in infants with complete congenital heart block of both the IgG and IgM classes.
• Synonyms: CRP55, Calregulin, HACBP, ERp60, grp60, CALR, CRTC
• Note: Molecular weight: 48142 Da

Reference Data

• Protein Families: Druggable Genome, Secreted Protein, Transcription Factors
• Protein Pathways: Antigen processing and presentation