alpha 1b Adrenergic Receptor (ADRA1B) (Center) Rabbit Polyclonal Antibody

CAT#: AP50099PU-N

alpha 1b Adrenergic Receptor (ADRA1B) (Center) rabbit polyclonal antibody, Aff - Purified

Product Images

Western blot analysis of ADRA1B Antibody (Center) in NCI-H460 cell line lysates (35ug/lane). ADRA1B (arrow) was detected using the purified Pab.

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  Formalin-fixed and paraffin-embedded human brain with ADRA1B Antibody (Center), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.

Specifications

Product Data

• Applications: IHC, WB
• Recommended Dilution: ELISA: 1/1000.
  Western blotting: 1/100 - 1/500.
  Immunohistochemistry: 1/50 - 1/100.
• Reactivities: Human
• Host: Rabbit
• Isotype: Ig
• Clonality: Polyclonal
• Immunogen: KLH conjugated synthetic peptide between 276~306 amino acids from the Central region of human ADRA1B
• Specificity: This antibody reacts to Alpha-1B adrenergic receptor.
• Formulation: PBS containing 0.09% (W/V) sodium azide as preservative
  State: Aff - Purified
  State: Liquid purified Ig fraction
• Concentration: lot specific
• Purification: Affinity chromatography on Protein A
• Storage: Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
  Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.
• Gene Name: Homo sapiens adrenoceptor alpha 1B (ADRA1B)
• Database Link:
  Entrez Gene 147 Human
• Background: Alpha-1-adrenergic receptors (alpha-1-ARs) are members of the G protein-coupled receptor superfamily. They activate mitogenic responses and regulate growth and proliferation of many cells. There are 3 alpha-1-AR subtypes: alpha-1A, -1B and -1D, all of which signal through the Gq/11 family of G-proteins and different subtypes show different patterns of activation. This protein encodes alpha-1B-adrenergic receptor, which induces neoplastic transformation when transfected into NIH 3T3 fibroblasts and other cell lines. Thus, this normal cellular gene is identified as a protooncogene. This protein comprises 2 exons and a single large intron of at least 20 kb that interrupts the coding region.
• Synonyms: Alpha 1B-adrenoreceptor, Alpha 1B-adrenoceptor
• Note: Molecular Weight: 56;836 Da

Reference Data

• Protein Families: Druggable Genome, GPCR, Transmembrane
• Protein Pathways: Calcium signaling pathway, Neuroactive ligand-receptor interaction, Vascular smooth muscle contraction