Salivary alpha amylase (AMY1A) (Center) Rabbit Polyclonal Antibody

CAT#: AP50166PU-N

Salivary alpha amylase (AMY1A) (Center) rabbit polyclonal antibody, Aff - Purified

Product Images

Western blot analysis of AMY1A Antibody (Center) in HL-60 cell line lysates (35ug/lane). AMY1A (arrow) was detected using the purified Pab.

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  Formalin-fixed and paraffin-embedded human prostate carcinoma reacted with AMY1A Antibody (Center), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.

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  AMY1A Antibody (Center) flow cytometric analysis of CEM cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

Specifications

Product Data

• Applications: FC, IHC, WB
• Recommended Dilution: ELISA: 1/1000.
  Western blotting: 1/100 - 1/500.
  Immunohistochemistry: 1/50 - 1/100.
  Flow cytometric: 1/10 - 1/50.
• Reactivities: Human
• Host: Rabbit
• Isotype: Ig
• Clonality: Polyclonal
• Immunogen: KLH conjugated synthetic peptide between 202~231 amino acids from the Central region of human AMY1A
• Specificity: This antibody reacts to Alpha-amylase 1.
• Formulation: PBS containing 0.09% (W/V) sodium azide as preservative
  State: Aff - Purified
  State: Liquid purified Ig fraction
• Concentration: lot specific
• Purification: Affinity chromatography on Protein A
• Storage: Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
  Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.
• Gene Name: Homo sapiens amylase, alpha 1B (salivary) (AMY1B)
• Database Link:
  Entrez Gene 277 HumanEntrez Gene 276 Human
• Background: Amylases are secreted proteins that hydrolyze 1,4-alpha-glucoside bonds in oligosaccharides and polysaccharides, and thus catalyze the first step in digestion of dietary starch and glycogen. The human genome has a cluster of several amylase genes that are expressed at high levels in either salivary gland or pancreas. It is an amylase isoenzyme produced by the salivary gland.
• Synonyms: Salivary alpha-amylase, AMY1A
• Note: Molecular Weight: 57768 Da

Reference Data

• Protein Pathways: Metabolic pathways, Starch and sucrose metabolism