ASXL1 (Center) Rabbit Polyclonal Antibody

CAT#: AP50279PU-N

ASXL1 (Center) rabbit polyclonal antibody, Aff - Purified

Product Images

Western blot analysis in mouse liver tissue lysates (35ug/lane) using ASXL1 antibody (Center).This demonstrates the ASXL1 antibody detected the ASXL1 protein (arrow).

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  Immunohistochemistry analysis in formalin fixed and paraffin embedded human testis tissue using ASXL1 Antibody followed by peroxidase conjugation of the secondary antibody and DAB staining.

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  Confocal immunofluorescent analysis of ASXL1 Antibody (Center) with Hela cell followed by Alexa Fluor 488-conjugated Goat anti-Rabbit lgG (green). Actin filaments have been labeled with Alexa Fluor555 phalloidin (red). DAPI was used to stain the cell nuclear (blue).

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  Flow Cytometric analysis of Hela cells (Right Histogram) using ASXL1 antibody (Center) compared to a negative control cell (Left Histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

Specifications

Product Data

• Applications: FC, IF, IHC, WB
• Recommended Dilution: ELISA: 1/1000.
  Western blotting: 1/100 - 1/500.
  Flow Cytometry: 1/10 - 1/50.
  Immunofluorescence: 1/10 - 1/50.
  Immunohistochemistry on Paraffin Sections: 1/50 - 1/100.
• Reactivities: Human, Mouse
• Host: Rabbit
• Isotype: Ig
• Clonality: Polyclonal
• Immunogen: KLH conjugated synthetic peptide between 521-549 amino acids from the Central region of human ASXL1
• Specificity: This antibody detects ASXL1 at Center.
• Formulation: PBS
  State: Aff - Purified
  State: Liquid purified Ig fraction
  Preservative: 0.09% Sodium Azide
• Concentration: lot specific
• Purification: Affinity Chromatography on Protein A
• Storage: Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
  Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.
• Predicted Protein Size: 165448 Da
• Gene Name: Homo sapiens additional sex combs like 1, transcriptional regulator (ASXL1), transcript variant 1
• Database Link:
  Entrez Gene 171023 Human
• Background: This gene is similar to the Drosophila additional sex combs gene, which encodes a chromatin-binding protein required for normal determination of segment identity in the developing embryo. The protein is a member of the Polycomb group of proteins, which are necessary for the maintenance of stable repression of homeotic and other loci. The protein is thought to disrupt chromatin in localized areas, enhancing transcription of certain genes while repressing the transcription of other genes. The protein encoded by this gene functions as a ligand-dependent co-activator for retinoic acid receptor in cooperation with nuclear receptor coactivator 1. Mutations in this gene are associated with myelodysplastic syndromes and chronic myelomonocytic leukemia. Alternative splicing results in multiple transcript variants.
• Synonyms: KIAA0978