BARD1 (N-term) Rabbit Polyclonal Antibody

CAT#: AP50339PU-N

BARD1 (N-term) rabbit polyclonal antibody, Aff - Purified

Product Images

BARD1 Antibody (N-term) western blot analysis in WiDr cell line lysates (35ug/lane).This demonstrates the BARD1 antibody detected the BARD1 protein (arrow).

Specifications

Product Data

• Applications: WB
• Recommended Dilution: ELISA: 1/1000.
  Western blotting: 1/100 - 1/500.
• Reactivities: Human
• Host: Rabbit
• Isotype: Ig
• Clonality: Polyclonal
• Immunogen: KLH conjugated synthetic peptide between 104-135 amino acids from the N-terminal region of human BARD1.
• Specificity: This antibody reacts to BARD1.
• Formulation: PBS containing 0.09% (W/V) sodium azide as preservative
  State: Aff - Purified
  State: Liquid purified Ig fraction
• Concentration: lot specific
• Purification: Affinity chromatography on Protein A
• Storage: Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
  Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.
• Gene Name: Homo sapiens BRCA1 associated RING domain 1 (BARD1), transcript variant 1
• Database Link:
  Entrez Gene 580 Human
• Background: BARD1 is a protein which interacts with the N-terminal region of BRCA1. In addition to its ability to bind BRCA1 in vivo and in vitro, it shares homology with the 2 most conserved regions of BRCA1: the N-terminal RING motif and the C-terminal BRCT domain. The RING motif is a cysteine-rich sequence found in a variety of proteins that regulate cell growth, including the products of tumor suppressor genes and dominant protooncogenes. This protein also contains 3 tandem ankyrin repeats. The BARD1/BRCA1 interaction is disrupted by tumorigenic amino acid substitutions in BRCA1, implying that the formation of a stable complex between these proteins may be an essential aspect of BRCA1 tumor suppression. This protein may be the target of oncogenic mutations in breast or ovarian cancer.
• Synonyms: BARD-1
• Note: Molecular Weight: 86648 Da

Reference Data

• Protein Families: Druggable Genome