BCL2A1 (Center) Rabbit Polyclonal Antibody

CAT#: AP50357PU-N

BCL2A1 (Center) rabbit polyclonal antibody, Purified

Product Images

Western blot analysis of BCL2A1 Antibody (Center) in T47D cell line lysates (35ug/lane). BCL2A1 (arrow) was detected using the purified Pab.

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  Formalin-fixed and paraffin-embedded human lung carcinoma reacted with BCL2A1 Antibody (Center), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.

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  BCL2A1 Antibody (Center) flow cytometric analysis of MDA-MB231 cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

Specifications

Product Data

• Applications: FC, IHC, WB
• Recommended Dilution: ELISA: 1/1000.
  Western blotting: 1/50 - 1/100.
  Immunohistochemistry on paraffin sections: 1/50 - 1/100.
  Flow cytometric: 1/10 - 1/50.
• Reactivities: Human
• Host: Rabbit
• Isotype: Ig
• Clonality: Polyclonal
• Immunogen: KLH conjugated synthetic peptide between 63~92 amino acids from the Center region of human BCL2A1
• Specificity: This antibody reacts to Bcl-2-like 5.
• Formulation: PBS containing 0.09% (W/V) sodium azide as preservative
  State: Purified
  State: Liquid purified Ig fraction
• Concentration: lot specific
• Purification: Affinity chromatography on Protein A
• Storage: Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
  Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.
• Gene Name: Homo sapiens BCL2 related protein A1 (BCL2A1), transcript variant 1
• Database Link:
  Entrez Gene 597 Human
• Background: Bcl-2 related Protein A1 is a member of the BCL-2 protein family. The proteins of this family form hetero- or homodimers and act as anti- and pro-apoptotic regulators that are involved in a wide variety of cellular activities such as embryonic development, homeostasis and tumorigenesis. The protein encoded by this gene is able to reduce the release of pro-apoptotic cytochrome c from mitochondria and block caspase activation. This gene is a direct transcription target of NF-kappa B in response to inflammatory mediators, and has been shown to be up-regulated by different extracellular signals, such as granulocyte-macrophage colony-stimulating factor (GM-CSF), CD40, phorbol ester and inflammatory cytokine TNF and IL-1, which suggests a cytoprotective function that is essential for lymphocyte activation as well as cell survival.
• Synonyms: BCL2A1, BCL2L5, BFL1, GRS, HBPA1, Bcl-2-like protein 5, Protein BFL-1
• Note: Molecular Weight: 20132 Da

Reference Data

• Protein Families: Druggable Genome
• Protein Pathways: Metabolic pathways