CAD (Center) Rabbit Polyclonal Antibody

CAT#: AP50710PU-N

CAD (Center) rabbit polyclonal antibody, Aff - Purified

Product Images

CAD Antibody (Center) western blot analysis in Jurkat cell line lysates (35ug/lane).This demonstrates the CAD antibody detected the CAD protein (arrow).

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  CAD Antibody (Center) immunohistochemistry analysis in formalin fixed and paraffin embedded human breast carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of CAD Antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.

Specifications

Product Data

• Applications: IHC, WB
• Recommended Dilution: ELISA: 1/1000.
  Western blot: 1/100 - 1/500.
  Immunohistochemistry on paraffin sections: 1/10 - 1/50.
• Reactivities: Human
• Host: Rabbit
• Isotype: Ig
• Clonality: Polyclonal
• Immunogen: KLH conjugated synthetic peptide between 787-816 amino acids from the Central region of human CAD
• Specificity: This antibody reacts to CAD.
• Formulation: PBS
  State: Aff - Purified
  State: Liquid purified Ig fraction
  Preservative: 0.09% (W/V) sodium azide
• Concentration: lot specific
• Purification: Affinity chromatography on Protein A
• Storage:

  Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
  Avoid repeated freezing and thawing.

• Stability: Shelf life: one year from despatch.

• Predicted Protein Size: 242984 Da
• Gene Name: Homo sapiens carbamoyl-phosphate synthetase 2, aspartate transcarbamylase, and dihydroorotase (CAD), transcript variant 1
• Database Link:
  Entrez Gene 790 Human
• Background: The de novo synthesis of pyrimidine nucleotides is required for mammalian cells to proliferate. This gene encodes a trifunctional protein which is associated with the enzymatic activities of the first 3 enzymes in the 6-step pathway of pyrimidine biosynthesis: carbamoylphosphate synthetase (CPS II), aspartate transcarbamoylase, and dihydroorotase. This protein is regulated by the mitogen-activated protein kinase (MAPK) cascade, which indicates a direct link between activation of the MAPK cascade and de novo biosynthesis of pyrimidine nucleotides.
• Synonyms: Dihydroorotase, GATase, ACTase, DHOase

Reference Data

• Protein Families: Druggable Genome
• Protein Pathways: Alanine, aspartate and glutamate metabolism, Metabolic pathways, Pyrimidine metabolism