Complement factor B (CFB) (Center) Rabbit Polyclonal Antibody

CAT#: AP50876PU-N

Complement factor B (CFB) (Center) rabbit polyclonal antibody, Aff - Purified

Product Images

Western blot analysis of CFB Antibody (Center) in mouse kidney tissue lysates (35ug/lane). CFB (arrow) was detected using the purified Pab.

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  CFB Antibody (Center) immunohistochemistry analysis in formalin fixed and paraffin embedded human skeletal muscle followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the CFB Antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.

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  CFB Antibody (Center) flow cytometric analysis of 293 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

Specifications

Product Data

• Applications: FC, IHC, WB
• Recommended Dilution: ELISA: 1/1000.
  Western Blot: 1/1000.
  Immunohistochemistry on paraffin sections: 1/50 - 1/100.
  Flow Cytometry: 1/10 - 1/50.
• Reactivities: Human, Mouse
• Host: Rabbit
• Isotype: Ig
• Clonality: Polyclonal
• Immunogen: KLH conjugated synthetic peptide between 469~494 amino acids from the Center region of human CFB
• Specificity: This antibody reacts to CFB.
• Formulation: PBS
  State: Aff - Purified
  State: Liquid purified Ig fraction
  Preservative: 0.09% (W/V) sodium azide
• Concentration: lot specific
• Purification: Affinity chromatography on Protein A
• Storage:

  Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
  Avoid repeated freezing and thawing.

• Stability: Shelf life: one year from despatch.

• Predicted Protein Size: 85533 Da
• Gene Name: Homo sapiens complement factor B (CFB)
• Database Link:
  Entrez Gene 14962 MouseEntrez Gene 629 Human
• Background: CFB encodes complement factor B, a component of the alternative pathway of complement activation. Factor B circulates in the blood as a single chain polypeptide. Upon activation of the alternative pathway, it is cleaved by complement factor D yielding the noncatalytic chain Ba and the catalytic subunit Bb. The active subunit Bb is a serine protease which associates with C3b to form the alternative pathway C3 convertase. Bb is involved in the proliferation of preactivated B lymphocytes, while Ba inhibits their proliferation.
• Synonyms: Properdin factor B, C3/C5 convertase, PBF2, CFB, BF, BFD

Reference Data

• Protein Families: Druggable Genome, Protease, Secreted Protein
• Protein Pathways: Complement and coagulation cascades