Choline Acetyltransferase (CHAT) (N-term) Rabbit Polyclonal Antibody

CAT#: AP50885PU-N

Choline Acetyltransferase (CHAT) (N-term) rabbit polyclonal antibody, Purified

Product Images

Western blot analysis of CHAT Antibody (N-term) in NCI-H460 cell line lysates (35ug/lane).CHAT (arrow) was detected using the purified Pab.

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  Western blot analysis of CHAT Antibody (N-term) in mouse liver tissue lysates (35ug/lane). CHAT (arrow) was detected using the purified Pab.

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  Confocal immunofluorescent analysis of CHAT Antibody (N-term) with NCI-H460 cell followed by Alexa Fluor® 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).

Specifications

Product Data

• Applications: IF, WB
• Recommended Dilution: Peptide ELISA: 1/1000.
  Western Blot: 1/1000.
  Immunofluorescence: 1/10 - 1/50.
• Reactivities: Human, Mouse
• Host: Rabbit
• Isotype: Ig
• Clonality: Polyclonal
• Immunogen: KLH conjugated synthetic peptide between 98-128 amino acids from the N-terminal region of human CHAT.
• Specificity: This antibody reacts to CHAT.
• Formulation: PBS
  State: Purified
  State: Liquid purified Ig fraction
  Preservative: 0.09% (W/V) sodium azide
• Concentration: lot specific
• Purification: Saturated Ammonium Sulfate (SAS) precipitation
• Storage:

  Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
  Avoid repeated freezing and thawing.

• Stability: Shelf life: one year from despatch.

• Predicted Protein Size: 82536 Da
• Gene Name: Homo sapiens choline O-acetyltransferase (CHAT), transcript variant M
• Database Link:
  Entrez Gene 12647 MouseEntrez Gene 1103 Human
• Background: CHAT is an enzyme which catalyzes the biosynthesis of the neurotransmitter acetylcholine. This gene product is a characteristic feature of cholinergic neurons, and changes in these neurons may explain some of the symptoms of Alzheimer disease.
• Synonyms: Choline O-acetyltransferase, Choline acetylase, CHOACTase, ChAT, EC=2.3.1.6

Reference Data

• Protein Families: Druggable Genome
• Protein Pathways: Glycerophospholipid metabolism