CHPF (Center) Rabbit Polyclonal Antibody

CAT#: AP50902PU-N

CHPF (Center) rabbit polyclonal antibody, Aff - Purified

Product Images

Western blot analysis of CHPF (arrow) in MDA-MB435, MCF-7, HepG2, A375 cell line and mouse testis tissue lysates (35ug/lane) using Chondroitin sulfate synthase 2 antibody.

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  Immunohistochemistry analysis of human testis tissue (formalin-fixed, paraffin-embedded) using Chondroitin sulfate synthase 2 antibody., which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for IHC; clinical relevance has not been evaluated.

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  Flow cytometry analysis of MCF-7 cells (bottom histogram) compared to a negative control cell (top histogram) using Chondroitin sulfate synthase 2 antibody., followed by FITC-conjugated goat-anti-rabbit secondary antibodies.

Specifications

Product Data

• Applications: FC, IHC, WB
• Recommended Dilution:

  This antibody is suitable for flow cytometry, immunohistochemistry on paraffin sections and Western blotting.
  The suggested dilution is:
  Flow cytometry: 1/10-1/50.
  Immunohistochemistry on paraffin sections: 1/50-1/100.
  Western blotting: 1/100-1/500.

• Reactivities: Human, Mouse
• Host: Rabbit
• Isotype: Ig
• Clonality: Polyclonal
• Immunogen: Synthetic peptide - KLH conjugated - corresponding to the center region of human Chondroitin sulfate synthase 2/CHPF.
• Specificity: This antibody detects Chondroitin sulfate synthase 2/CHPF.
• Formulation: PBS with 0.09% (W/V) Sodium Azide.
  State: Aff - Purified
  State: Liquid purified Ig fraction
• Concentration: lot specific
• Purification: Purified through a protein A column; followed by peptide affinity purification.
• Storage:

  Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
  Avoid repeated freezing and thawing.

• Stability: Shelf life: one year from despatch.

• Gene Name: Homo sapiens chondroitin polymerizing factor (CHPF), transcript variant 2
• Database Link:
  Entrez Gene 74241 MouseEntrez Gene 79586 Human
• Background: CHPF is a protein that has both beta-1,3-glucuronic acid and beta-1,4-N-acetylgalactosamine transferase activity. Transfers glucuronic acid (GlcUA) from UDP-GlcUA and N-acetylgalactosamine (GalNAc) from UDP-GalNAc to the non-reducing end of the elongating chondroitin polymer.
• Synonyms: CHPF, CSS2
• Note: Molecular Weight: 85495 Da

Reference Data

• Protein Families: Druggable Genome
• Protein Pathways: Chondroitin sulfate biosynthesis, Metabolic pathways