Endostatin (COL18A1) (Center) Rabbit Polyclonal Antibody

CAT#: AP51011PU-N

Endostatin (COL18A1) (Center) rabbit polyclonal antibody, Aff - Purified

Product Images

Western blot analysis in mouse liver tissue lysates (35ug/lane) using COL18A1 Antibody (Center) Cat.-No AP51011PU-N.This demonstrates the COL18A1 antibody detected the COL18A1 protein (arrow).

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  Formalin fixed and paraffin embedded human hepatocarcinoma stained with COL18A1 Antibody (Center) Cat.-No AP51011PU-N followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the COL18A1 Antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.

Specifications

Product Data

• Applications: IHC, WB
• Recommended Dilution: ELISA: 1/1000.
  Western blot: 1/1000.
  Immunohistochemistry: 1/50-100.
• Reactivities: Human, Mouse
• Host: Rabbit
• Isotype: Ig
• Clonality: Polyclonal
• Immunogen: KLH conjugated synthetic peptide between 1311-1339 amino acids from the Central region of Human COL18A1.
• Specificity: This antibody recognizes COL18A1 Antibody (Center).
• Formulation: PBS
  State: Aff - Purified
  State: Liquid purified Ig fraction
  Preservative: 0.09% (W/V) Sodium Azide
• Concentration: lot specific
• Purification: Protein A column, followed by peptide affinity purification
• Storage:

  Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
  Avoid repeated freezing and thawing.

• Stability: Shelf life: one year from despatch.

• Predicted Protein Size: 178188 Da
• Gene Name: Homo sapiens collagen type XVIII alpha 1 (COL18A1), transcript variant 1
• Database Link:
  Entrez Gene 12822 MouseEntrez Gene 80781 Human
• Background: COL18A1 is the alpha chain of type XVIII collagen. This collagen is one of the multiplexins, extracellular matrix proteins that contain multiple triple-helix domains (collagenous domains) interrupted by non-collagenous domains. The proteolytically produced C-terminal fragment of type XVIII collagen is endostatin, a potent antiangiogenic protein. Mutations in this gene are associated with Knobloch syndrome. The main features of this syndrome involve retinal abnormalities, so type XVIII collagen may play an important role in retinal structure and in neural tube closure.
• Synonyms: COL18A1, Endostatin, KNO1