CPSF30 (CPSF4) (Center) Rabbit Polyclonal Antibody

CAT#: AP51059PU-N

CPSF30 (CPSF4) (Center) rabbit polyclonal antibody, Aff - Purified

Product Images

Western blot analysis of CPSF4 Antibody (Center) in Jurkat cell line lysates (35ug/lane).CPSF4 (arrow) was detected using the purified Pab.

Specifications

Product Data

• Applications: WB
• Recommended Dilution: ELISA: 1/1000.
  Western Blot: 1/1000.
• Reactivities: Human
• Host: Rabbit
• Isotype: Ig
• Clonality: Polyclonal
• Immunogen: KLH conjugated synthetic peptide between 96~123 amino acids from the Central region of Human CPSF4.
• Specificity: This antibody recognizes CPSF4 (Center).
• Formulation: PBS
  State: Aff - Purified
  State: Liquid purified Ig fraction
  Preservative: 0.09% Sodium Azide
• Concentration: lot specific
• Purification: Affinity Chromatography on Protein A
• Storage:

  Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
  Avoid repeated freezing and thawing.

• Stability: Shelf life: one year from despatch.

• Gene Name: Homo sapiens cleavage and polyadenylation specific factor 4 (CPSF4), transcript variant 2
• Database Link:
  Entrez Gene 10898 Human
• Background:
  Inhibition of the nuclear export of poly(A)-containing mRNAs caused by the influenza A virus NS1 protein requires its effector domain. The NS1 effector domain functionally interacts with the cellular 30 kDa subunit of cleavage and polyadenylation specific factor 4, an essential component of the 3' end processing machinery of cellular pre-mRNAs. In influenza virus-infected cells, the NS1 protein is physically associated with cleavage and polyadenylation specific factor 4, 30kD subunit. Binding of the NS1 protein to the 30 kDa protein in vitro prevents CPSF binding to the RNA substrate and inhibits 3' end cleavage and polyadenylation of host pre-mRNAs. Thus the NS1 protein selectively inhibits the nuclear export of cellular, and not viral, mRNAs.
• Synonyms: CPSF30, NAR, NEB1, No arches homolog
• Note: Molecular Weight: 30255 Da