CXXC4 (C-term) Rabbit Polyclonal Antibody

CAT#: AP51153PU-N

CXXC4 (C-term) rabbit polyclonal antibody, Aff - Purified

Product Images

Western blot analysis in K562 cell line lysates (35ug/lane) using CXXC4 antibody. This demonstrates the CXXC4 antibody detected the CXXC4 protein (arrow).

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  Western blot analysis of CXXC4 antibody. in mouse testis tissue lysates (35ug/lane).

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  Formalin-fixed, paraffin-embedded human colon carcinoma reacted with CXXC4 antibody. (C-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.

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  Flow cytometry analysis of K562 cells (bottom histogram) compared to a negative control cell (top histogram) using CXXC4 antibody.FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

Specifications

Product Data

• Applications: FC, IHC, WB
• Recommended Dilution: This antibody is suitable for flow cytometry, immonohistochemistry on paraffin sections and Western blotting.
  The suggested dilution is:
  Flow cytometry: 1/10-1/50.
  Immunohistochemistry on paraffin sections: 1/50-1/100.
  Western blotting: 1/1001/500.
• Reactivities: Human
• Host: Rabbit
• Isotype: Ig
• Clonality: Polyclonal
• Immunogen: Synthetic peptide - KLH conjugated - corresponding to the C-terminal region (between 164-194aa) of human CXXC4.
• Specificity: This antibody detects human CXXC4 at C-term.
• Formulation: PBS with 0.09% (W/V) Sodium Azide.
  State: Aff - Purified
  State: Liquid purified Ig fraction
• Concentration: lot specific
• Purification: Purified through a protein A column, followed by peptide affinity purification.
• Storage:

  Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
  Avoid repeated freezing and thawing.

• Stability: Shelf life: one year from despatch.

• Gene Name: Homo sapiens CXXC finger protein 4 (CXXC4), transcript variant 1
• Database Link:
  Entrez Gene 80319 Human
• Background: CXXC4 acts as a negative regulator of the Wnt signaling pathway via its interaction with DVL1.
• Synonyms: IDAX
• Note: Molecular Weight: 20978 Da

Reference Data

• Protein Families: Druggable Genome
• Protein Pathways: Wnt signaling pathway