CYP2A7 (Center) Rabbit Polyclonal Antibody

CAT#: AP51169PU-N

CYP2A7 (Center) rabbit polyclonal antibody, Aff - Purified

Product Images

Western blot analysis in MDA-MB231 cell line lysates (35ug/lane) using CYP2A7 antibody. This demonstrates the CYP2A7 antibody detected the CYP2A7 protein (arrow).

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  Immunohistochemistry analysis in human hepatocarcinoma tissue (Formalin-fixed, Paraffin-embedded) using CYP2A7 antibody., followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the CYP2A7 antibody for IHC; Clinical relevance has not been evaluated.

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  Flow cytometric analysis of MDA-MB231 cells (right histogram) compared to a negative control cell (left histogram) using CYP2A7 antibody., followed by FITC-conjugated goat-anti-rabbit secondary antibodies.

Specifications

Product Data

• Applications: FC, IHC, WB
• Recommended Dilution:

  Flow cytometry: 1/10-1/50.
  Immunohistochemistry on Paraffin sections: 1/50-1/100.
  Western blot: 1/100-1/500.
  Enzyme immunoassay: 1/1000.

• Reactivities: Human
• Host: Rabbit
• Isotype: Ig
• Clonality: Polyclonal
• Immunogen: Synthetic peptide - KLH conjugated - corresponding to the central regio (between 128-15aa) of human CYP2A7.
• Specificity: This antibody recognizes CYP2A7.
• Formulation: PBS with 0.09% (W/V) Sodium azide
  State: Aff - Purified
  State: Liquid purified Ig fraction
• Concentration: lot specific
• Purification: Purified through a Protein A column followed by peptide affinity purification
• Storage:

  Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
  Avoid repeated freezing and thawing.

• Stability: Shelf life: one year from despatch.

• Gene Name: Homo sapiens cytochrome P450 family 2 subfamily A member 7 (CYP2A7), transcript variant 1
• Database Link:
  Entrez Gene 1549 Human
• Background: CYP2A7 is a member of the cytochrome P450 superfamily of enzymes. The cytochrome P450 proteins are monooxygenases which catalyze many reactions involved in drug metabolism and synthesis of cholesterol, steroids and other lipids. This protein localizes to the endoplasmic reticulum; its substrate has not yet been determined. CYP2A7, which produces two transcript variants, is part of a large cluster of cytochrome P450 genes from the CYP2A, CYP2B and CYP2F subfamilies on chromosome 19q.
• Synonyms: Cytochrome P450 2A7, CYPIIA7, Cytochrome P450-IIA4
• Note: Molecular Weight: 56425 Da

Reference Data

• Protein Families: Druggable Genome, P450, Transmembrane
• Protein Pathways: Caffeine metabolism, Drug metabolism - cytochrome P450, Drug metabolism - other enzymes, Metabolic pathways, Retinol metabolism