Dynamitin (DCTN2) (Center) Rabbit Polyclonal Antibody

CAT#: AP51207PU-N

Dynamitin (DCTN2) (Center) rabbit polyclonal antibody, Purified

Product Images

Western blot analysis of DCTN2 (arrow) using Dynactin subunit 2 Antibody. 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the DCTN2 gene.

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  Formalin fixed and paraffin embedded human stomach tissue stained with Dynactin subunit 2 Antibody followed by peroxidase conjugation of the secondary antibody and DAB staining

Specifications

Product Data

• Applications: IHC, WB
• Recommended Dilution: ELISA: 1/1000.
  Western Blot: 1/100-1/500.
  Immunohistochemistry on Paraffin Sections: 1/10-1/50.
• Reactivities: Human
• Host: Rabbit
• Isotype: Ig
• Clonality: Polyclonal
• Immunogen: KLH conjugated synthetic peptide between 191-219 amino acids from the Central region of Human Dynactin subunit 2.
• Specificity: This antibody recognizes Human Dynactin subunit 2 (Center).
• Formulation: PBS
  State: Purified
  State: Liquid purified Ig fraction
  Preservative: 0.09% Sodium Azide
• Concentration: lot specific
• Purification: Protein A column, followed by peptide affinity purification
• Storage:

  Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
  Avoid repeated freezing and thawing.

• Stability: Shelf life: one year from despatch.

• Gene Name: Homo sapiens dynactin subunit 2 (DCTN2), transcript variant 1
• Database Link:
  Entrez Gene 10540 Human
• Background:
  This gene encodes a 50-kD subunit of dynactin, a macromolecular complex consisting of 10-11 subunits ranging in size from 22 to 150 kD. Dynactin binds to both microtubules and cytoplasmic dynein. It is involved in a diverse array of cellular functions, including ER-to-Golgi transport, the centripetal movement of lysosomes and endosomes, spindle formation, chromosome movement, nuclear positioning, and axonogenesis. This subunit is present in 4-5 copies per dynactin molecule. It contains three short alpha-helical coiled-coil domains that may mediate association with self or other dynactin subunits. It may interact directly with the largest subunit (p150) of dynactin and may affix p150 in place.
• Synonyms: DCTN2, DCTN50, Dynactin 2, p50 dynamitin, RBP50
• Note: Molecular Weight: 44231 Da

Reference Data

• Protein Pathways: Huntington's disease