DFFB (N-term) Rabbit Polyclonal Antibody

CAT#: AP51245PU-N

DFFB (N-term) rabbit polyclonal antibody, Aff - Purified

Product Images

Western blot analysis of DFFB / CAD Antibody (N-term) in 293 cell line lysates (35ug/lane). DFFB (arrow) was detected using the purified Pab.

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  Formalin fixed and paraffin embedded prostate carcinoma reacted with DFFB / CAD Antibody (N-term) followed by peroxidase conjugation of the secondary antibody and DAB staining.

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  Flow cytometric analysis of 293 cells using DFFB / CAD Antibody (N-term) (right histogram) compared to a negative control cell (left histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

Specifications

Product Data

• Applications: FC, IHC, WB
• Recommended Dilution:

  ELISA: 1/1000.
  Western Blot: 1/100-1/500.
  Flow Cytometry: 1/10-1/50.
  Immunohistochemistry on Paraffin Sections: 1/50-1/100.

• Reactivities: Human
• Host: Rabbit
• Isotype: Ig
• Clonality: Polyclonal
• Immunogen: KLH conjugated synthetic peptide between 1~30 amino acids from the N-terminal region of Human DFFB / CAD.
• Specificity: This antibody recognizes Human DFFB / CAD (N-term).
• Formulation: PBS
  State: Aff - Purified
  State: Liquid purified Ig fraction
  Preservative: 0.09% Sodium Azide
• Concentration: lot specific
• Purification: Affinity Chromatography on Protein A
• Storage:

  Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
  Avoid repeated freezing and thawing.

• Stability: Shelf life: one year from despatch.

• Gene Name: Homo sapiens DNA fragmentation factor subunit beta (DFFB), transcript variant 2
• Database Link:
  Entrez Gene 1677 Human
• Background: Apoptosis is a cell death process that removes toxic and/or useless cells during mammalian development. The apoptotic process is accompanied by shrinkage and fragmentation of the cells and nuclei and degradation of the chromosomal DNA into nucleosomal units. DNA fragmentation factor (DFF) is a heterodimeric protein of 40-kD (DFFB) and 45-kD (DFFA) subunits. DFFA is the substrate for caspase-3 and triggers DNA fragmentation during apoptosis. DFF becomes activated when DFFA is cleaved by caspase-3. The cleaved fragments of DFFA dissociate from DFFB, the active component of DFF. DFFB has been found to trigger both DNA fragmentation and chromatin condensation during apoptosis
• Synonyms: DFF2, CPAN, DFF-40, DFF40
• Note: Molecular Weight: 39110 Da

Reference Data

• Protein Families: Druggable Genome
• Protein Pathways: Apoptosis