DIO3 (C-term) Rabbit Polyclonal Antibody

CAT#: AP51263PU-N

DIO3 (C-term) rabbit polyclonal antibody, Aff - Purified

Product Images

Western blot analysis of DIO3 Antibody (C-term) in HepG2 cell line lysates (35ug/lane). DIO3 (arrow) was detected using the purified Pab.

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  Formalin-fixed and paraffin-embedded human hepatocarcinoma reacted with DIO3 Antibody (C-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.

Specifications

Product Data

• Applications: IHC, WB
• Recommended Dilution: ELISA: 1/1000.
  Western Blot: 1/100-1/500.
  Immunohistochemistry on Paraffin Sections: 1/50-1/100.
  
• Reactivities: Human
• Host: Rabbit
• Isotype: Ig
• Clonality: Polyclonal
• Immunogen: KLH conjugated synthetic peptide between 249~278 amino acids from the C-terminal region of human DIO3
• Specificity: This antibody recognizes Human DIO3 (C-term).
• Formulation: PBS
  State: Aff - Purified
  State: Liquid purified Ig fraction
  Preservative: 0.09% Sodium Azide
• Concentration: lot specific
• Purification: Affinity Chromatography on Protein A
• Storage:

  Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
  Avoid repeated freezing and thawing.

• Stability: Shelf life: one year from despatch.

• Gene Name: Homo sapiens deiodinase, iodothyronine, type III (DIO3)
• Database Link:
  Entrez Gene 1735 Human
• Background: DIO3 belongs to the iodothyronine deiodinase family. It catalyzes the inactivation of thyroid hormone by inner ring deiodination of the prohormone thyroxine (T4) and the bioactive hormone 3,3',5-triiodothyronine (T3) to inactive metabolites, 3,3',5'-triiodothyronine (RT3) and 3,3'-diiodothyronine (T2), respectively. This enzyme is highly expressed in the pregnant uterus, placenta, fetal and neonatal tissues, suggesting that it plays an essential role in the regulation of thyroid hormone inactivation during embryological development. This protein contains a selenocysteine (Sec) residue, which is essential for efficient enzyme activity. The selenocysteine is encoded by the UGA codon, which normally signals translation termination. The 3' UTR of Sec-containing genes have a common stem-loop structure, the sec insertion sequence (SECIS), which is necessary for the recognition of UGA as a Sec codon rather than as a stop signal.
• Synonyms: ITDI3, TXDI3, Type 3 DI, DIOIII, 5DIII
• Note: Molecular Weight: 31451 Da

Reference Data

• Protein Families: Druggable Genome