DNAJB6 (Center) Rabbit Polyclonal Antibody

CAT#: AP51288PU-N

DNAJB6 (Center) rabbit polyclonal antibody, Aff - Purified

Product Images

Western blot analysis of DNAJB6 (arrow) pre-incubated without (lane 1) and with (lane 2) blocking peptide in Hela cell line lysate using DNAJB6 antibody. (1:60/250 dilution).

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  Immunohistochemistry analysis in human brain tissue (Formalin-fixed, Paraffin-embedded) using DNAJB6 antibody., followed by peroxidase conjugated secondary antibody and DAB staining. This data demonstrates the use of this antibody for IHC. Clinical relevance has not been evaluated.

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  Flow cytometric analysis of Hela cells (right histogram) compared to a negative control cell (left histogram) using DNAJB6 antibody., followed by FITC-conjugated goat-anti-rabbit secondary antibodies.

Specifications

Product Data

• Applications: FC, IHC, WB
• Recommended Dilution:

  Flow cytometry: 1/10-1/50.
  Immunohistochemistry on Paraffin sections: 1/10-1/50.
  Western blot: 1/100-1/500.
  Enzyme immunoassay: 1/1000.

• Reactivities: Human
• Host: Rabbit
• Isotype: Ig
• Clonality: Polyclonal
• Immunogen: Synthetic peptide - KLH conjugated - corresponding to the central region (between 217-247aa) of human DNAJB6
• Specificity: This antibody recognizes DNAJB6.
• Formulation: PBS with 0.09% (W/V) Sodium azide
  State: Aff - Purified
  State: Liquid purified Ig fraction
• Concentration: lot specific
• Purification: Purified through a Protein A column followed by peptide affinity purification
• Storage:

  Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
  Avoid repeated freezing and thawing.

• Stability: Shelf life: one year from despatch.

• Gene Name: Homo sapiens DnaJ heat shock protein family (Hsp40) member B6 (DNAJB6), transcript variant 2
• Database Link:
  Entrez Gene 10049 Human
• Background: The DNAJB6 gene encodes a member of the DNAJ protein family. DNAJ family members are characterized by a highly conserved amino acid stretch called the 'J-domain' and function as one of the two major classes of molecular chaperones involved in a wide range of cellular events, such as protein folding and oligomeric protein complex assembly. This family member may also play a role in polyglutamine aggregation in specific neurons. Alternative splicing of this gene results in multiple transcript variants; however, not all variants have been fully described.
• Synonyms: Heat shock protein J2, MSJ-1, HHDJ1, MRJ, HSJ2, MRJ, MSJ1
• Note: Molecular Weight: 36087 Da