Endothelin 1 (EDN1) (Center) Rabbit Polyclonal Antibody

CAT#: AP51364PU-N

Endothelin 1 (EDN1) (Center) rabbit polyclonal antibody, Aff - Purified

Product Images

Western blot analysis of Endothelin-1 Antibody in A549 cell line lysates (35ug/lane).

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  Formalin fixed and paraffin embedded human placenta tissue reacted with Endothelin-1 Antibody followed by peroxidase conjugation of the secondary antibody and DAB staining.

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  Confocal immunofluorescent analysis of Endothelin-1 Antibody with A549 cell and followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).

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  Flow cytometric analysis of A549 cells using Endothelin-1 Antibody (right histogram) compared to a negative control cell (left histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

Specifications

Product Data

• Applications: FC, IF, IHC, WB
• Recommended Dilution: ELISA: 1/1000.
  Western Blot: 1/1000.
  Flow Cytometry: 1/10-1/50.
  Immunofluorescence: 1/10-1/50.
  Immunohistochemistry on Paraffin Sections: 1/50-1/100.
• Reactivities: Human
• Host: Rabbit
• Isotype: Ig
• Clonality: Polyclonal
• Immunogen: KLH conjugated synthetic peptide between 111-138 amino acids from the Central region of human EDN1
• Specificity: This antibody recognizes Human Endothelin-1 (Center).
• Formulation: PBS containing 0.09% (W/V) Sodium Azide as preservative
  State: Aff - Purified
  State: Liquid purified Ig fraction
• Concentration: lot specific
• Purification: Affinity Chromatography on Protein A
• Storage:

  Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
  Avoid repeated freezing and thawing.

• Stability: Shelf life: one year from despatch.

• Gene Name: Homo sapiens endothelin 1 (EDN1), transcript variant 2
• Database Link:
  Entrez Gene 1906 Human
• Background:
  The protein encoded by this gene is proteolytically processed to release a secreted peptide termed endothelin 1. This peptide is a potent vasoconstrictor and is produced by vascular endothelial cells. Endothelin 1 also can affect the central nervous system. Two transcript variants encoding different isoforms have been found for this gene.
• Synonyms: PPET1, EDN1, ET1, HDLCQ7, EDN1
• Note: Molecular Weight: 24425 Da

Reference Data

• Protein Families: Druggable Genome, Secreted Protein
• Protein Pathways: Melanogenesis