Endothelin 1 (EDN1) (C-term) Rabbit Polyclonal Antibody

CAT#: AP51365PU-N

Endothelin 1 (EDN1) (C-term) rabbit polyclonal antibody, Aff - Purified

Product Images

Western blot analysis of Endothelin-1 Antibody (C-term) in ZR-75-1, U251, MDA-MB453, A549 cell line lysates (35ug/lane). This demonstrates the EDN1 antibody detected the EDN1 protein (arrow).

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  Formalin fixed and paraffin embedded human liver tissue reacted with Endothelin-1 Antibody (C-term) followed by peroxidase conjugation of the secondary antibody and DAB staining.

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  Confocal immunofluorescent analysis of Endothelin-1 Antibody (C-term) with A549 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).

Specifications

Product Data

• Applications: IF, IHC, WB
• Recommended Dilution: ELISA: 1/1000.
  Western Blot: 1/100-1/500.
  Immunofluorescence: 1/10-1/50.
  Immunohistochemistry on Paraffin Sections: 1/50-1/100.
• Reactivities: Human
• Host: Rabbit
• Isotype: Ig
• Clonality: Polyclonal
• Immunogen: KLH conjugated synthetic peptide between 183-211 amino acids from the C-terminal region of Human EDN1
• Specificity: This antibody recognizes Human Endothelin-1 (C-term).
  Other species not tested.
• Formulation: PBS containing 0.09% (W/V) Sodium Azide as preservative
  State: Aff - Purified
  State: Liquid purified Ig fraction
• Concentration: lot specific
• Purification: Affinity Chromatography on Protein A
• Storage:

  Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
  Avoid repeated freezing and thawing.

• Stability: Shelf life: one year from despatch.

• Gene Name: Homo sapiens endothelin 1 (EDN1), transcript variant 2
• Database Link:
  Entrez Gene 1906 Human
• Background: The protein encoded by this gene is proteolytically processed to release a secreted peptide termed endothelin 1. This peptide is a potent vasoconstrictor and is produced by vascular endothelial cells. Endothelin 1 also can affect the central nervous system. Two transcript variants encoding different isoforms have been found for this gene.
• Synonyms: PPET1, EDN1, ET1, HDLCQ7, EDN1
• Note: Molecular Weight: 24425 Da

Reference Data

• Protein Families: Druggable Genome, Secreted Protein
• Protein Pathways: Melanogenesis