EIF2A (Center) Rabbit Polyclonal Antibody

CAT#: AP51390PU-N

EIF2A (Center) rabbit polyclonal antibody, Aff - Purified

Product Images

Western blot analysis of EIF2A Antibody (Center) in Ramos cell line lysates (35ug/lane). EIF2A (arrow) was detected using the purified Pab.

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  Formalin-fixed and paraffin-embedded human brain tissue reacted with EIF2A Antibody (Center), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.

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  EIF2A Antibody (Center) flow cytometry analysis of Ramos cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

Specifications

Product Data

• Applications: FC, IHC, WB
• Recommended Dilution: ELISA: 1/1000.
  Western Blot: 1/100 - 1/500.
  Immunofluorescence: 1/50 - 1/100.
  Flow Cytometry: 1/10 - 1/50.
• Reactivities: Human
• Host: Rabbit
• Isotype: Ig
• Clonality: Polyclonal
• Immunogen: KLH conjugated synthetic peptide between 444~473 amino acids from the Central region of human EIF2A
• Specificity: This antibody reacts to EIF2A.
• Formulation: PBS
  State: Aff - Purified
  State: Liquid purified Ig fraction
  Preservative: 0.09% (W/V) sodium azide
• Concentration: lot specific
• Purification: Affinity chromatography on Protein A
• Storage:

  Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
  Avoid repeated freezing and thawing.

• Stability: Shelf life: one year from despatch.

• Predicted Protein Size: 64990 Da
• Gene Name: Homo sapiens eukaryotic translation initiation factor 2A (EIF2A), transcript variant 1
• Database Link:
  Entrez Gene 83939 Human
• Background: EIF2A is a 65-kD protein that catalyzes the formation of puromycin-sensitive 80S preinitiation complexes (Zoll et al., 2002 [PubMed 12133843]).
• Synonyms: eIF-2A, Eukaryotic translation initiation factor 2A, CDA02, MSTP004, MSTP089