EIF3S3 (EIF3H) (N-term) Rabbit Polyclonal Antibody

CAT#: AP51397PU-N

EIF3S3 (EIF3H) (N-term) rabbit polyclonal antibody, Aff - Purified

Product Images

Western blot analysis of EIF3H (arrow) using rabbit polyclonal EIF3H Antibody (N-term). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the EIF3H gene.

• 

  EIF3H Antibody (N-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human hepatocarcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of EIF3H Antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated.

Specifications

Product Data

• Applications: IHC, WB
• Recommended Dilution: ELISA: 1/1000.
  Western Blot: 1/100 - 1/500.
  Immunohistochemistry on paraffin sections: 1/10 - 1/50.
• Reactivities: Human
• Host: Rabbit
• Isotype: Ig
• Clonality: Polyclonal
• Immunogen: KLH conjugated synthetic peptide between 69-99 amino acids from the N-terminal region of human EIF3H
• Specificity: This antibody reacts to EIF3H.
• Formulation: PBS
  State: Aff - Purified
  State: Liquid purified Ig fraction
  Preservative: 0.09% (W/V) sodium azide
• Concentration: lot specific
• Purification: Affinity chromatography on Protein A
• Storage:

  Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
  Avoid repeated freezing and thawing.

• Stability: Shelf life: one year from despatch.

• Predicted Protein Size: 39930 Da
• Gene Name: Homo sapiens eukaryotic translation initiation factor 3 subunit H (EIF3H)
• Database Link:
  Entrez Gene 8667 Human
• Background: Component of the eukaryotic translation initiation factor 3 (eIF-3) complex, which is required for several steps in the initiation of protein synthesis. The eIF-3 complex associates with the 40S ribosome and facilitates the recruitment of eIF-1, eIF-1A, eIF-2:GTP:methionyl-tRNAi and eIF-5 to form the 43S preinitiation complex (43S PIC). The eIF-3 complex stimulates mRNA recruitment to the 43S PIC and scanning of the mRNA for AUG recognition. The eIF-3 complex is also required for disassembly and recycling of posttermination ribosomal complexes and subsequently prevents premature joining of the 40S and 60S ribosomal subunits prior to initiation.
• Synonyms: Eukaryotic translation initiation factor 3 subunit H, eIF-3 gamma, eIF3 p40