ENGASE (Center) Rabbit Polyclonal Antibody

CAT#: AP51428PU-N

ENGASE (Center) rabbit polyclonal antibody, Aff - Purified

Product Images

Western blot analysis of ENASE Antibody in HL-60 cell line lysates (35ug/lane). This demonstrates the ENASE antibody detected the ENASE protein (arrow).

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  Formalin fixed and paraffin embedded human spleen tissue reacted with ENASE Antibody followed by peroxidase conjugation of the secondary antibody and DAB staining.

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  Flow cytometric analysis of HL-60 cells using ENASE Antibody (right histogram) compared to a negative control cell (left histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

Specifications

Product Data

• Applications: FC, IHC, WB
• Recommended Dilution: ELISA: 1/1000.
  Western Blot: 1/100-1/500.
  Flow Cytometry: 1/10-1/50.
  Immunohistochemistry on Paraffin Sections: 1/50-1/100.
• Reactivities: Human
• Host: Rabbit
• Isotype: Ig
• Clonality: Polyclonal
• Immunogen: KLH conjugated synthetic peptide between 326-354 amino acids from the Central region of Human ENASE
• Specificity: This antibody recognizes Human ENASE (Center).
• Formulation: PBS containing 0.09% (W/V) Sodium Azide as preservative
  State: Aff - Purified
  State: Liquid purified Ig fraction
• Concentration: lot specific
• Purification: Affinity Chromatography on Protein A
• Storage:

  Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
  Avoid repeated freezing and thawing.

• Stability: Shelf life: one year from despatch.

• Gene Name: Homo sapiens endo-beta-N-acetylglucosaminidase (ENGASE)
• Database Link:
  Entrez Gene 64772 Human
• Background: Endo-beta-N-acetylglucosaminidase (ENGase; EC 3.2.1.96) is involved in the processing of free oligosaccharides in the cytosol.
• Synonyms: Cytosolic endo-beta-N-acetylglucosaminidase
• Note: Molecular Weight: 83987 Da

Reference Data

• Protein Pathways: Other glycan degradation