HIF 2 alpha (EPAS1) (N-term) Rabbit Polyclonal Antibody

CAT#: AP51440PU-N

HIF 2 alpha (EPAS1) (N-term) rabbit polyclonal antibody, Aff - Purified

Product Images

Western blot analysis of HIF2A / HIF2 alpha Antibody (N-term) in A549 cell line lysates (35ug/lane). This demonstrates the EPAS1 antibody detected the EPAS1 protein (arrow).

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  Flow cytometric analysis of A549 cells using HIF2A / HIF2 alpha Antibody (N-term) (right histogram) compared to a negative control cell (left histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

Specifications

Product Data

• Applications: FC, WB
• Recommended Dilution: ELISA: 1/1,000.
  Western Blot: 1/100-1/500.
  Flow Cytometry: 1/10-1/50.
• Reactivities: Human
• Host: Rabbit
• Isotype: Ig
• Clonality: Polyclonal
• Immunogen: KLH conjugated synthetic peptide between 127-154 amino acids from the N-terminal region of Human HIF2A
• Specificity: This antibody recognizes Human HIF2A / HIF2 alpha (N-term).
• Formulation: PBS containing 0.09% (W/V) Sodium Azide as preservative
  State: Aff - Purified
  State: Liquid purified Ig fraction
• Concentration: lot specific
• Purification: Affinity Chromatography on Protein A
• Storage:

  Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
  Avoid repeated freezing and thawing.

• Stability: Shelf life: one year from despatch.

• Gene Name: Homo sapiens endothelial PAS domain protein 1 (EPAS1)
• Database Link:
  Entrez Gene 2034 Human
• Background: This gene encodes a transcription factor involved in the induction of genes regulated by oxygen, which is induced as oxygen levels fall. The encoded protein contains a basic-helix-loop-helix domain protein dimerization domain as well as a domain found in proteins in signal transduction pathways which respond to oxygen levels. Mutations in this gene are associated with erythrocytosis familial type 4.
• Synonyms: BHLHE73, HIF-2 alpha, MOP2, PASD2, Hypoxia-inducible factor 2 alpha, HIF-1 alpha-like factor, HLF, HIF-related factor, EPAS1
• Note: Molecular Weight: 96459 Da

Reference Data

• Protein Families: Druggable Genome, Transcription Factors
• Protein Pathways: Pathways in cancer, Renal cell carcinoma