ETFA (C-term) Rabbit Polyclonal Antibody

CAT#: AP51468PU-N

ETFA (C-term) rabbit polyclonal antibody, Aff - Purified

Product Images

Western blot analysis of ETFA Antibody (C-term) in K562 cell line and mouse liver tissue lysates (35ug/lane). This demonstrates the ETFA antibody detected the ETFA protein (arrow).

• 

  Formalin fixed and paraffin embedded human skeletal muscle reacted with ETFA Antibody (C-term) followed by peroxidase conjugation of the secondary antibody and DAB staining.

• 

  Flow cytometric analysis of Ramos cells using ETFA Antibody (C-term) (right histogram) compared to a negative control cell (left histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

Specifications

Product Data

• Applications: FC, IHC, WB
• Recommended Dilution: ELISA: 1/1000.
  Western Blot: 1/100-1/500.
  Flow Cytometry: 1/10-1/50.
  Immunohistochemistry on Paraffin Sections: 1/50-1/100.
• Reactivities: Human, Mouse
• Host: Rabbit
• Isotype: Ig
• Clonality: Polyclonal
• Immunogen: KLH conjugated synthetic peptide between 283-311 amino acids from the C-terminal region of human ETFA
• Specificity: This antibody recognizes Human and Mouse ETFA (C-term).
• Formulation: PBS containing 0.09% (W/V) Sodium Azide as preservative
  State: Aff - Purified
  State: Liquid purified Ig fraction
• Concentration: lot specific
• Purification: Affinity Chromatography on Protein A
• Storage:

  Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
  Avoid repeated freezing and thawing.

• Stability: Shelf life: one year from despatch.

• Gene Name: Homo sapiens electron transfer flavoprotein alpha subunit (ETFA), transcript variant 1
• Database Link:
  Entrez Gene 110842 MouseEntrez Gene 2108 Human
• Background: ETFA participates in catalyzing the initial step of the mitochondrial fatty acid beta-oxidation. It shuttles electrons between primary flavoprotein dehydrogenases and the membrane-bound electron transfer flavoprotein ubiquinone oxidoreductase. Defects in electron-transfer-flavoprotein have been implicated in type II glutaricaciduria in which multiple acyl-CoA dehydrogenase deficiencies result in large excretion of glutaric, lactic, ethylmalonic, butyric, isobutyric, 2-methyl-butyric, and isovaleric acids. Two transcript variants encoding different isoforms have been found for this gene.
• Synonyms: Alpha-ETF
• Note: Molecular Weight: 35080 Da

Reference Data

• Protein Families: Druggable Genome